Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/35361
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Mendes, Gabriel | por |
dc.contributor.author | Vieira, Pedro S. | por |
dc.contributor.author | Lanceros-Méndez, S. | por |
dc.contributor.author | Kluskens, Leon | por |
dc.contributor.author | Mota, M. | por |
dc.date.accessioned | 2015-06-01T15:13:33Z | - |
dc.date.available | 2015-06-01T15:13:33Z | - |
dc.date.issued | 2015-08 | - |
dc.date.submitted | 2015-04 | - |
dc.identifier.citation | Mendes, Gabriel Pinto; Vieira, Pedro S.; Lanceros-Mendez, S.; Kluskens, Leon; Mota, M., Transformation of Escherichia coli JM109 using pUC19 by the Yoshida effect. Journal of Microbiological Methods, 115, 1-5, 2015 | por |
dc.identifier.issn | 0167-7012 | por |
dc.identifier.uri | https://hdl.handle.net/1822/35361 | - |
dc.description.abstract | Transformation of non-competent Escherichia coli JM109 was accomplished using pUC19 as donor plasmid and sepiolite as the acicular material to promote cell piercing via application of friction with a polystyrene stick or a magnetic bar on the surface of a hydrogel containing agar. An automatic spreading setup was built with a conventional stirring plate and compared to manual spreading. Several parameters were optimized, namely, the agar content of the hydrogel (2%), concentration of cells (OD=1.3 corresponding to 1.4x109 bacterial cells/mL), concentration of sepiolite (0.01%), manual versus mechanical spreading (automatic spreading more consistent) and spreading time (30 sec). Efficiency values up to 4.1 x 104 CFU/µg pUC19 were obtained. The method proved to be suitable for a rapid and low cost transformation of non-competent E. coli JM109, where higher values of efficiency do not need to be attained. | por |
dc.description.sponsorship | Portuguese Foundation for Scienceand Technology (FCT) and the European Community fund FEDER,through Program COMPETE, under the scope of the Projects FCOMP-01-0124-FEDER-007025 (PTDC/AMB/68393/2006), PEst-OE/EQB/LA0023/ 2013, PEST-C/FIS/UI607/2013, RECI/BBB-EBI/0179/2012 (FCOMP-01- 0124-FEDER-027462) and the Projects “BioEnv — Biotechnology and Bioengineering for a sustainableworld” and “Matepro—Optimizing Materials and Processes”. NORTE-07-0124-FEDER-000048 was co-funded by the Programa Operacional Regional do Norte (ON.2 — O Novo Norte), QREN, FEDER. The authors also acknowledge the fellowship awarded to Gabriel Mendes SFRH/BD/71661/2010 under the scope of the MITPortugal Program. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier B.V. | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/68393/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/3599-PPCDT/132966/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/COMPETE/132974/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/126270/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F71661%2F2010/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/COMPETE/126270/PT | - |
dc.rights | openAccess | por |
dc.subject | Yoshida effect | por |
dc.subject | Transformation | por |
dc.subject | Sepiolite | por |
dc.subject | E. coli | por |
dc.title | Transformation of Escherichia coli JM109 using pUC19 by the Yoshida effect | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0167701215001517 | por |
dc.comments | CEB21044 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 5 | por |
oaire.citationConferencePlace | Netherlands | - |
oaire.citationTitle | Journal of Microbiological Methods | por |
oaire.citationVolume | 115 | por |
dc.date.updated | 2015-06-01T08:16:20Z | - |
dc.identifier.eissn | 0167-7012 | - |
dc.identifier.doi | 10.1016/j.mimet.2015.05.012 | por |
dc.identifier.pmid | 25966644 | por |
dc.subject.fos | Ciências Médicas::Biotecnologia Médica | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Microbiological Methods | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_21044_1.pdf | 1,01 MB | Adobe PDF | Ver/Abrir |