Utilize este identificador para referenciar este registo: http://hdl.handle.net/1822/33050

TítuloWater and carbon dioxide : green solvents for the extraction of collagen/gelatin from marine sponges
Autor(es)Barros, Alexandre A.
Aroso, Ivo Manuel Ascensão
Silva, Tiago H.
Mano, J. F.
Duarte, Ana Rita C.
Reis, R. L.
Palavras-chaveGreen solvents
Extraction
Collagen
Gelatin
Marine sponges
Carbon dioxide
Water
Natural products
Data23-Dez-2014
EditoraAcs Publications
RevistaACS Sustainable Chemistry and Engineering
Citação2. Barros, A. A., Aroso, I. M., Silva, T. H., Mano, J. F., Duarte, A. R. C., & Reis, R. L. (2015). Water and Carbon Dioxide: Green Solvents for the Extraction of Collagen/Gelatin from Marine Sponges. Acs Sustainable Chemistry & Engineering, 3(2), 254-260. doi: 10.1021/sc500621z
Resumo(s)Marine sponges are extremely rich in natural products and are considered a promising biological resource. The major objective of this work is to couple a green extraction process with a natural origin raw material to obtain sponge origin collagen/gelatin for biomedical applications. Marine sponge collagen has unique physicochemical properties, but its application is hindered by the lack of availability due to inefficient extraction methodologies. Traditional extraction methods are time consuming as they involve several operating steps and large amounts of solvents. In this work, we propose a new extraction methodology under mild operating conditions in which water is acidified with carbon dioxide (CO2) to promote the extraction of collagen/gelatin from different marine sponge species. An extraction yield of approximately 50% of collagen/gelatin was achieved. The results of Fourier transformed infrared spectroscopy (FTIR), circular dichroism (CD), and differential scanning calorimetry (DSC) spectra suggest a mixture of collagen/gelatin with high purity, and the analysis of the amino acid composition has shown similarities with collagen from other marine sources. Additionally, in vitro cytotoxicity studies did not demonstrate any toxicity effects for three of the extracts.
Tipoarticle
Descrição"Publication Date (Web): December 23, 2014"
URIhttp://hdl.handle.net/1822/33050
DOI10.1021/sc500621z
ISSN2168-0485
Versão da editorahttp://pubs.acs.org/doi/abs/10.1021/sc500621z
Arbitragem científicayes
AcessoopenAccess
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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