Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/33050
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Barros, Alexandre A. | por |
dc.contributor.author | Aroso, Ivo Manuel Ascensão | por |
dc.contributor.author | Silva, Tiago H. | por |
dc.contributor.author | Mano, J. F. | por |
dc.contributor.author | Duarte, Ana Rita C. | por |
dc.contributor.author | Reis, R. L. | por |
dc.date.accessioned | 2015-01-21T10:39:52Z | - |
dc.date.available | 2015-01-21T10:39:52Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Barros, A. A., Aroso, I. M., Silva, T. H., Mano, J. F., Duarte, A. R. C., & Reis, R. L. (2015). Water and Carbon Dioxide: Green Solvents for the Extraction of Collagen/Gelatin from Marine Sponges. Acs Sustainable Chemistry & Engineering, 3(2), 254-260. doi: 10.1021/sc500621z | por |
dc.identifier.issn | 2168-0485 | por |
dc.identifier.uri | https://hdl.handle.net/1822/33050 | - |
dc.description | "Publication Date (Web): December 23, 2014" | por |
dc.description.abstract | Marine sponges are extremely rich in natural products and are considered a promising biological resource. The major objective of this work is to couple a green extraction process with a natural origin raw material to obtain sponge origin collagen/gelatin for biomedical applications. Marine sponge collagen has unique physicochemical properties, but its application is hindered by the lack of availability due to inefficient extraction methodologies. Traditional extraction methods are time consuming as they involve several operating steps and large amounts of solvents. In this work, we propose a new extraction methodology under mild operating conditions in which water is acidified with carbon dioxide (CO2) to promote the extraction of collagen/gelatin from different marine sponge species. An extraction yield of approximately 50% of collagen/gelatin was achieved. The results of Fourier transformed infrared spectroscopy (FTIR), circular dichroism (CD), and differential scanning calorimetry (DSC) spectra suggest a mixture of collagen/gelatin with high purity, and the analysis of the amino acid composition has shown similarities with collagen from other marine sources. Additionally, in vitro cytotoxicity studies did not demonstrate any toxicity effects for three of the extracts. | por |
dc.description.sponsorship | The authors are grateful for financial support of FCT through Grant EXP/QEQ:EPS/0745/2012, SWIMS (Subcritical Water Isolation of compounds from Marine Sponges). The funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement numbers REGPOT-CT2012-316331-POLARIS and KBBE-2010-266033 (project SPECIAL), as well as from ERDF under the project "Novel smart and biomimetic materials for innovative regenerative medicine approaches" RLI-ABMR-NORTE-01-0124-FEDER-000016), cofinanced by North Portugal Regional Operational Programme (ON.2,O Novo Norte), under the National Strategic Reference Framework (NSRF) are also gratefully ackowledged. The authors are also truly thankfull to Prof. Micha flan (Tel Aviv University, Israel), Dr. Ronald Osinga (Porifarma, The Netherlands), Dr. Antonio Sara and Dr. Martina Milanese (Studio Associato GAIA, Italy), and Dr. Joana Xavier (University of Azores) for the kind offer of marine sponges samples. | por |
dc.language.iso | eng | por |
dc.publisher | ACS Publications | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/127219/PT | - |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/316331/EU | - |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/266033/EU | - |
dc.relation | info:eu-repo/grantAgreement/FCT/COMPETE/127219/PT | - |
dc.rights | openAccess | por |
dc.subject | Green solvents | por |
dc.subject | Extraction | por |
dc.subject | Collagen | por |
dc.subject | Gelatin | por |
dc.subject | Marine sponges | por |
dc.subject | Carbon dioxide | por |
dc.subject | Water | por |
dc.subject | Natural products | por |
dc.title | Water and carbon dioxide: green solvents for the extraction of collagen/gelatin from marine sponges | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://pubs.acs.org/doi/abs/10.1021/sc500621z | por |
dc.comments | http://www.3bs.uminho.pt/node/18293 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 254 | por |
oaire.citationEndPage | 260 | por |
oaire.citationIssue | 2 | por |
oaire.citationTitle | ACS sustainable chemistry & engineering | por |
oaire.citationVolume | 3 | por |
dc.date.updated | 2015-01-16T15:43:36Z | - |
dc.identifier.doi | 10.1021/sc500621z | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | ACS Sustainable Chemistry and Engineering | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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18293-Alex.pdf | 4,5 MB | Adobe PDF | Ver/Abrir |