Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/32980

TitleEvaluation of the effects of selected phytochemicals on quorum sensing inhibition and in vitro cytotoxicity
Author(s)Borges, Anabela
Serra, Sofia Cristina
Abreu, Ana Cristina
Saavedra, Maria J.
Salgado, A. J.
Simões, Manuel
KeywordsBiofilms
Chromobacterium violaceum
Citotoxicity
Isothiocyanates
Phenolics
Phytochemicals
Quorum sensing inhibition
cytotoxicity
Issue date2014
PublisherTaylor & Francis
JournalBiofouling
Abstract(s)Quorum sensing (QS) is an important regulatory mechanism in biofilm formation and differentiation. Interference with QS can affect biofilm development and antimicrobial susceptibility. This study evaluates the potential of selected phytochemical products to inhibit QS. Three isothiocyanates (allylisothiocyanate - AITC, benzylisothiocyanate - BITC and 2-phenylethylisothiocyanate - PEITC) and six phenolic products (gallic acid - GA, ferulic acid - FA, caffeic acid - CA, phloridzin - PHL, (-) epicatechin - EPI and oleuropein glucoside - OG) were tested. A disc diffusion assay based on pigment inhibition in Chromobacterium violaceum CV12472 was performed. In addition, the mechanisms of QS inhibition (QSI) based on the modulation of N-acyl homoserine lactone (AHLs) activity and synthesis by the phytochemicals were investigated. The cytotoxicity of each product was tested on a cell line of mouse lung fibroblasts. AITC, BITC and PEITC demonstrated a capacity for QSI by modulation of AHL activity and synthesis, interfering the with QS systems of C. violaceum CviI/CviR homologs of LuxI/LuxR systems. The cytotoxic assays demonstrated low effects on the metabolic viability of the fibroblast cell line only for FA, PHL and EPI.
TypeArticle
URIhttp://hdl.handle.net/1822/32980
DOI10.1080/08927014.2013.852542
ISSN0892-7014
1029-2454
Publisher versionhttp://dx.doi.org/10.1080/08927014.2013.852542
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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