Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/32898

TitleDifferential sensitivities to lactate transport inhibitors of breast cancer cell lines
Author(s)Santos, Filipa Morais
Gonçalves, Vera M.
Pinheiro, Sílvia
Vieira, André Filipe
Paredes, Joana
Schmitt, Fernando C.
Baltazar, Fátima
Pinheiro, Céline
KeywordsBreast
Molecular biology
Carcinoma
Issue date2014
PublisherSociety for Endocrinology
JournalEndocrine-Related Cancer
Abstract(s)The tumour microenvironment is known to be acidic due to high glycolytic rates of tumour cells. Monocarboxylate transporters (MCTs) play a role in extracellular acidification, which is widely known to be involved in tumour progression. Recently, we have described the upregulation of MCT1 in breast carcinomas and its association with poor prognostic variables. Thus, we aimed to evaluate the effect of lactate transport inhibition in human breast cancer cell lines. The effects of a-cyano-4-hydroxycinnamate, quercetin and lonidamine on cell viability, metabolism, proliferation, apoptosis, migration and invasion were assessed in a panel of different breast cancer cell lines. MCT1, MCT4 and CD147 were differently expressed among the breast cancer cell lines and, as expected, different sensitivities were observed for the three inhibitors. Interestingly, in the most sensitive cell lines, lactate transport inhibition induced a decrease in cell proliferation, migration and invasion, as well as an increase in cell death. Results were validated by silencing MCT1 expression using siRNA. The results obtained here support targeting of lactate transport as a strategy to treat breast cancer, with a special emphasis on the basal-like subtype, which so far does not have a specific molecular therapy.
TypeArticle
DescriptionThis is linked to the online version of the paper at: http://dx.doi.org/10.1530/ERC-13-0132.
URIhttp://hdl.handle.net/1822/32898
DOI10.1530/ERC-13-0132
ISSN1351-0088
Publisher versionhttp://erc.endocrinology-journals.org/cgi/pmidlookup?view=long&pmid=24174370
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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