Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/31864

TitleDetection and discrimination of microorganisms using Locked Nucleic Acid - Fluorescence In Situ Hybridization (LNA-FISH)
Author(s)Azevedo, A. S.
Pereira, B.
Almeida, Carina
Melo, Luís F.
Wengel, J.
Azevedo, N. F.
Issue date2014
CitationAzevedo, A. S.; Pereira, B.; Almeida, Carina; Melo, L.; Wengel, J.; Azevedo, N. F., Detection and discrimination of microorganisms using Locked Nucleic Acid - Fluorescence In Situ Hybridization (LNA-FISH). CHEMPOR 2014 - Book of Extended Abstracts of the 12th International Chemical and Biological Engineering Conference. No. O-BE12, Porto, Portugal, Sep. 10-12, 3-32-3-342014. . ISBN: 978-972-752-170-8
Abstract(s)The impact of multispecies biofilms on catheter-associated urinary tract infections outcome is still unclear due to the lack of adequate methodologies to discriminate the populations in situ. Employing fluorescence in situ hybridization (FISH) to discriminate the populations in a biofilm, can contribute to the understanding of microorganisms interactions in this structures. Consequently, this information might help to develop efficient strategies to prevent this disease. This work presents the first study that apply the FISH methodology using a set of LNA and/2’-O-Methyl RNA oligonucleotide probes, for the in situ detection of microorganisms in biofilms formed under conditions similar to the catheter-associated urinary tract infections.
TypeAbstract
URIhttp://hdl.handle.net/1822/31864
ISBN978-972-752-170-8
Publisher versionhttp://chempor.ordemengenheiros.pt
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

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