Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/31647

TitleApplication of benzo[a]phenoxazinium chlorides in antimicrobial photodynamic therapy of Candida albicans biofilms
Author(s)Lopes, Marisa da Conceição Gomes
Alves, Carlos
Raju, B. Rama
Gonçalves, M. Sameiro T.
Coutinho, Paulo J. G.
Henriques, Mariana
Belo, Isabel
KeywordsCandida albicans
Biofilms
Antimicrobial photodynamic therapy
Benzo[a]phenoxazines
Issue date2014
PublisherElsevier B.V.
JournalJournal of Photochemistry and Photobiology B : Biology
CitationMarisa Lopes; Alves, C. T.; Rama Raju, B.; Gonçalves, M. S. T.; Coutinho, P. J. G.; Henriques, Mariana; Belo, Isabel, Application of benzo[a]phenoxazinium chlorides in antimicrobial photodynamic therapy of Candida albicans biofilms. Journal of Photochemistry and Photobiology B: Biology, 141, 93-99, 2014
Abstract(s)The use of Antimicrobial Photodynamic Therapy (APDT) as a new approach to treat localized Candida infections is an emerging and promising field nowadays. The aim of this study was to verify the efficacy of photodynamic therapy using two new benzo[a]phenoxazinium photosensitizers against Candida albicans biofilms: N-[5-(3-hydroxypropylamino)-10-methyl-9H-benzo[a]phenoxazin-9-ylidene]ethanaminium chloride (FSc) and N-(5-(11-hydroxyundecylamino)-10-methyl-9H-benzo[a]phenoxazin-9-ylidene)ethanaminium chloride (FSd). The photodynamic activity of dyes against C. albicans biofilms was evaluated by incubating biofilms with dyes in the range of 100-300 μM for 3 or 18 h followed by illumination at 12 or 36 J cm-2, using a xenon arc lamp (600 ± 2 nm). A total photoinactivation of C. albicans biofilm cells was achieved using 300 μM of FSc with18 h of incubation, followed by illumination at 36 J cm-2. Contrarily, FSd had insignificant effect on biofilms inactivation by APDT. The higher uptake of FSc than FSd dye by biofilms during the dark incubation may explain the greater photodynamic effectiveness achieved with FSc. The results obtained stresses out the FSc-mediated APDT potential use to treat C. albicans infections.
TypeArticle
URIhttp://hdl.handle.net/1822/31647
DOI10.1016/j.jphotobiol.2014.09.006
ISSN1873-2682
e-ISSN1011-1344
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
document_18064_1.pdf793,42 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID