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https://hdl.handle.net/1822/31647
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Campo DC | Valor | Idioma |
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dc.contributor.author | Lopes, Marisa da Conceição Gomes | por |
dc.contributor.author | Alves, Carlos | por |
dc.contributor.author | Raju, B. Rama | por |
dc.contributor.author | Gonçalves, M. Sameiro T. | por |
dc.contributor.author | Coutinho, Paulo J. G. | por |
dc.contributor.author | Henriques, Mariana | por |
dc.contributor.author | Belo, Isabel | por |
dc.date.accessioned | 2014-12-05T11:13:38Z | - |
dc.date.available | 2014-12-05T11:13:38Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Marisa Lopes; Alves, C. T.; Rama Raju, B.; Gonçalves, M. S. T.; Coutinho, P. J. G.; Henriques, Mariana; Belo, Isabel, Application of benzo[a]phenoxazinium chlorides in antimicrobial photodynamic therapy of Candida albicans biofilms. Journal of Photochemistry and Photobiology B: Biology, 141, 93-99, 2014 | por |
dc.identifier.issn | 1873-2682 | por |
dc.identifier.uri | https://hdl.handle.net/1822/31647 | - |
dc.description.abstract | The use of Antimicrobial Photodynamic Therapy (APDT) as a new approach to treat localized Candida infections is an emerging and promising field nowadays. The aim of this study was to verify the efficacy of photodynamic therapy using two new benzo[a]phenoxazinium photosensitizers against Candida albicans biofilms: N-[5-(3-hydroxypropylamino)-10-methyl-9H-benzo[a]phenoxazin-9-ylidene]ethanaminium chloride (FSc) and N-(5-(11-hydroxyundecylamino)-10-methyl-9H-benzo[a]phenoxazin-9-ylidene)ethanaminium chloride (FSd). The photodynamic activity of dyes against C. albicans biofilms was evaluated by incubating biofilms with dyes in the range of 100-300 μM for 3 or 18 h followed by illumination at 12 or 36 J cm-2, using a xenon arc lamp (600 ± 2 nm). A total photoinactivation of C. albicans biofilm cells was achieved using 300 μM of FSc with18 h of incubation, followed by illumination at 36 J cm-2. Contrarily, FSd had insignificant effect on biofilms inactivation by APDT. The higher uptake of FSc than FSd dye by biofilms during the dark incubation may explain the greater photodynamic effectiveness achieved with FSc. The results obtained stresses out the FSc-mediated APDT potential use to treat C. albicans infections. | por |
dc.description.sponsorship | This work was supported by the research grant SFRH/BD/72742/2010 from "Fundacao para a Ciencia e Tecnologia" (FCT), Portugal. The authors also thank the Project "BioHealth - Biotechnology and Bioengineering approaches to improve health quality", Ref. NORTE-07-0124-FEDER-000027, co-funded by the Programa Operacional Regional do Norte (ON.2 - 0 Novo Norte), QREN, FEDER and the FCT Strategic Project PEst-OE/EQB/LA0023/2013. In addition, financial to Centre of Chemistry and Centre of Physics through CQ/UM [PEst-C/QUI/UI0686/2013 (FCOMP-01-0124FEDER-037302)] and CFUM [PEst-C/FIS/UI0607/2013 (F-COMP-01-0124-FEDER-037291)], as well as a post-doctoral grant to B.R. Raju (SFRH/BPD/62881/2009) is also acknowledged to FCT, POPH-QREN, FSE. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier B.V. | por |
dc.rights | openAccess | por |
dc.subject | Candida albicans | por |
dc.subject | Biofilms | por |
dc.subject | Antimicrobial photodynamic therapy | por |
dc.subject | Benzo[a]phenoxazines | por |
dc.title | Application of benzo[a]phenoxazinium chlorides in antimicrobial photodynamic therapy of Candida albicans biofilms | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.comments | CEB18064 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 93 | por |
oaire.citationEndPage | 99 | por |
oaire.citationConferencePlace | Netherlands | - |
oaire.citationTitle | Journal of Photochemistry and Photobiology B : Biology | por |
oaire.citationVolume | 141 | por |
dc.date.updated | 2014-11-28T17:45:29Z | - |
dc.identifier.eissn | 1011-1344 | - |
dc.identifier.doi | 10.1016/j.jphotobiol.2014.09.006 | por |
dc.identifier.pmid | 25463655 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Photochemistry and Photobiology B: Biology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_18064_1.pdf | 793,42 kB | Adobe PDF | Ver/Abrir |