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TitleHigh level biosynthesis of a silk-elastin-like protein in E. coli
Author(s)Collins, Tony
Barroca, Mário Jorge Faria
Branca, Fernando
Padrão, Jorge
Machado, Raul
Casal, Margarida
Issue date3-Jun-2014
PublisherACS Publications
CitationHigh Level Biosynthesis of a Silk-Elastin-like Protein in E. coli Tony Collins, Mário Barroca, Fernando Branca, Jorge Padrão, Raul Machado, and Margarida Casal Biomacromolecules 2014 15 (7), 2701-2708
Abstract(s)Silk-elastin-like proteins (SELPs) have enormous potential for use as customizable biomaterials in numerous biomedical and materials applications yet success in harnessing this potential has been limited by the lack of a commercially viable industrially relevant production process. We have developed a scalable fed-batch production approach which enables a SELP volumetric productivity of 4.3 g L-1 with E. coli BL21(DE3). This is the highest SELP productivity reported to date and is 50-fold higher than that reported by other groups. As compared to typical fed-batch processes, high pre-induction growth rates and low inducer and oxygen concentrations are allowed whereas reduced post-induction feeding rates are preferred. Limiting factors were identified and productivity was found to be strongly influenced by a trade-off between the rate of production and plasmid stability. The process developed is robust, reproducible and applicable to scale up to the industrial level and moves these biopolymers a step closer to the marketplace.
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