Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/31031
Título: | High level biosynthesis of a silk-elastin-like protein in E. coli |
Autor(es): | Collins, Tony Barroca, Mário Jorge Faria Branca, Fernando Padrão, Jorge Machado, Raul Casal, Margarida |
Data: | 3-Jun-2014 |
Editora: | ACS Publications |
Revista: | Biomacromolecules |
Citação: | High Level Biosynthesis of a Silk-Elastin-like Protein in E. coli Tony Collins, Mário Barroca, Fernando Branca, Jorge Padrão, Raul Machado, and Margarida Casal Biomacromolecules 2014 15 (7), 2701-2708 |
Resumo(s): | Silk-elastin-like proteins (SELPs) have enormous potential for use as customizable biomaterials in numerous biomedical and materials applications yet success in harnessing this potential has been limited by the lack of a commercially viable industrially relevant production process. We have developed a scalable fed-batch production approach which enables a SELP volumetric productivity of 4.3 g L-1 with E. coli BL21(DE3). This is the highest SELP productivity reported to date and is 50-fold higher than that reported by other groups. As compared to typical fed-batch processes, high pre-induction growth rates and low inducer and oxygen concentrations are allowed whereas reduced post-induction feeding rates are preferred. Limiting factors were identified and productivity was found to be strongly influenced by a trade-off between the rate of production and plasmid stability. The process developed is robust, reproducible and applicable to scale up to the industrial level and moves these biopolymers a step closer to the marketplace. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/31031 |
DOI: | 10.1021/bm5005564 |
ISSN: | 1525-7797 1526-4602 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Collins et al, Biomacromolecules 2014 Final Published Version.pdf Acesso restrito! | 2,17 MB | Adobe PDF | Ver/Abrir |