Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/31031
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Campo DC | Valor | Idioma |
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dc.contributor.author | Collins, Tony | por |
dc.contributor.author | Barroca, Mário Jorge Faria | por |
dc.contributor.author | Branca, Fernando | por |
dc.contributor.author | Padrão, Jorge | por |
dc.contributor.author | Machado, Raul | por |
dc.contributor.author | Casal, Margarida | por |
dc.date.accessioned | 2014-11-20T10:23:54Z | - |
dc.date.available | 2014-11-20T10:23:54Z | - |
dc.date.issued | 2014-06-03 | - |
dc.identifier.citation | High Level Biosynthesis of a Silk-Elastin-like Protein in E. coli Tony Collins, Mário Barroca, Fernando Branca, Jorge Padrão, Raul Machado, and Margarida Casal Biomacromolecules 2014 15 (7), 2701-2708 | por |
dc.identifier.issn | 1525-7797 | por |
dc.identifier.issn | 1526-4602 | por |
dc.identifier.uri | https://hdl.handle.net/1822/31031 | - |
dc.description.abstract | Silk-elastin-like proteins (SELPs) have enormous potential for use as customizable biomaterials in numerous biomedical and materials applications yet success in harnessing this potential has been limited by the lack of a commercially viable industrially relevant production process. We have developed a scalable fed-batch production approach which enables a SELP volumetric productivity of 4.3 g L-1 with E. coli BL21(DE3). This is the highest SELP productivity reported to date and is 50-fold higher than that reported by other groups. As compared to typical fed-batch processes, high pre-induction growth rates and low inducer and oxygen concentrations are allowed whereas reduced post-induction feeding rates are preferred. Limiting factors were identified and productivity was found to be strongly influenced by a trade-off between the rate of production and plasmid stability. The process developed is robust, reproducible and applicable to scale up to the industrial level and moves these biopolymers a step closer to the marketplace. | por |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (FCT) | por |
dc.description.sponsorship | This work was financed by the European Commission, via the 7th Framework Programme Project EcoPlast (FP7-NMP-2009- SME-3, collaborative project number 246176), by FEDER through POFC − COMPETE and by national funds from Fundação para a Ciência e Tecnologia (FCT) through PEst- OE/BIA/U14050/2014. RM acknowledges FCT for SFRHBPD/ 86470/2012 grant. TC is thankful to FCT for its support through Programa Ciência 2008. | por |
dc.language.iso | eng | por |
dc.publisher | ACS Publications | por |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/246176 | por |
dc.rights | restrictedAccess | por |
dc.title | High level biosynthesis of a silk-elastin-like protein in E. coli | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 2701 | por |
oaire.citationEndPage | 2708 | por |
oaire.citationIssue | 7 | por |
oaire.citationTitle | Biomacromolecules | por |
oaire.citationVolume | 15 | por |
dc.identifier.doi | 10.1021/bm5005564 | por |
dc.identifier.pmid | 24884240 | por |
dc.subject.fos | Ciências Naturais::Ciências Biológicas | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biomacromolecules | por |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Collins et al, Biomacromolecules 2014 Final Published Version.pdf Acesso restrito! | 2,17 MB | Adobe PDF | Ver/Abrir |