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dc.contributor.authorSamy, S. M.-
dc.contributor.authorSilva, Nuno A.-
dc.contributor.authorCorrelo, Vitor M.-
dc.contributor.authorFraga, Joana S.-
dc.contributor.authorPinto, Luísa-
dc.contributor.authorTeixeira-Castro, Andreia-
dc.contributor.authorLeite-Almeida, Hugo-
dc.contributor.authorAlmeida, Armando-
dc.contributor.authorGimble, Jeffrey M.-
dc.contributor.authorSousa, Nuno-
dc.contributor.authorSalgado, António J.-
dc.contributor.authorReis, R. L.-
dc.description.abstractSpinal cord injury (SCI) leads to devastating neurological deficits. Several tissue engineering (TE)- based approaches have been investigated for repairing this condition. Poly (3-hydroxybutyrateco- 3-hydroxyvalerate) (PHB-HV) is found to be particularly attractive for TE applications due to its properties, such as biodegradability, biocompatibility, thermoplasticity and piezoelectricity. Hence, this report addresses the development and characterization of PHB-HV-based 3D scaffolds, produced by freeze-drying, aimed to SCI treatment. The obtained scaffolds reveal an anisotropic morphology with a fully interconnected network of pores. In vitro studies demonstrate a lack of cytotoxic effect of PHB-HV scaffolds. Direct contact assays also reveal their ability to support the culture of CNS-derived cells and mesenchymal-like stem cells from different sources. Finally, histocompatibility studies show that PHB-HV scaffolds are well tolerated by the host tissue, and do not negatively impact the left hindlimb locomotor function recovery. Therefore results herein presented suggest that PHB-HV scaffolds may be suitable for SCI treatment.por
dc.description.sponsorshipThis study was supported by the Portuguese Foundation for Science and Technology (FCT; Grant no PTDC/SAU-BMA/114059/2009; PEst-C/SAU/LA0001/2013-2014 and RNEM-REDE/1506/REM/2005) and Foundation Calouste Gulbenkian, under the scope of the Gulbenkian Program to Support Cutting Edge Research in Life Sciences (A.J.S.). This work was also partially supported by the European FP7 Project Find and Bind (NMP4-SL-2009-229292). The authors would like to thank Miguel Carvalho, Fabio Teixeira, and Filipa Campos for their collaboration in in vivo experiments.por
dc.subjectcell-based therapypor
dc.subjectPHB-HV 3D scaffoldspor
dc.subjectspinal cord injury regenerationpor
dc.subjecttissue engineeringpor
dc.titleDevelopment and characterization of PHB-HV based 3D scaffolds for a tissue engineering and cell-therapy combinatorial approach for spinal cord Injury regenerationpor
oaire.citationTitleMacromolecular Biosciencepor
dc.subject.wosScience & Technologypor
sdum.journalMacromolecular Biosciencepor
Appears in Collections:ICVS - Artigos em revistas internacionais / Papers in international journals

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