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Universidade do Minho > Escola de Engenharia da Universidade do Minho | School of Engineering at the University of Minho > Centro de Engenharia Biológica | Centre of Biological Engineering > CEB - Artigos em Revistas/Séries Internacionais / Papers in International Journals/Series >

Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/24933

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Title: Silver colloidal nanoparticles : effect on matrix composition and structure of Candida albicans and Candida glabrata biofilms
Authors: Monteiro, D. R.
Silva, Sónia Carina
Negri, M.
Gorup, L. F.
Camargo, E. R.
Oliveira, Rosário
Barbosa, D. B.
Henriques, Mariana
Keywords: Biofilms
Candida albicans
Candida glabrata
Fungal infections
Silver nanoparticles
Issue date: 2013
Publisher: Wiley
Abstract: Aim : The aim of this study was to assess the effect of different silver nanoparticles (SN) concentrations on the matrix composition and structure of Candida albicans and Candida glabrata biofilms. Methods and Results : Candida biofilms were developed in 6-well microtiter plates during 48 h. After, these biofilms were exposed to 13·5 or 54 μg SN ml−1 for 24 h. Then, extracellular matrices were extracted from biofilms and analysed chemically in terms of proteins, carbohydrates and DNA. To investigate the biofilm structure, scanning electron microscopy (SEM) and epifluorescence microscopy were used. SN interfered with the matrix composition of Candida biofilms tested in terms of protein, carbohydrate and DNA, except for the protein content of C. albicans biofilm. By SEM, Candida biofilms treated with SN revealed structural differences, when compared with the control groups. Further, SN showed a trend of agglomeration within the biofilms. Epifluorescence microscopy images suggest that SN induced damage on cell walls of the Candida isolates tested. Conclusions : In general, irrespective of concentration, SN affected the matrix composition and structure of Candida biofilms and these findings may be related to the mechanisms of biocide action of SN. Significance and Impact of the Study : This study reveals new insights about the behaviour of SN when in contact with Candida biofilms. SN may contribute to the development of therapies to prevent or control Candida infections.
Type: article
URI: http://hdl.handle.net/1822/24933
DOI: 10.1111/jam.12102
ISSN: 1364-5072
Peer-Reviewed: yes
Appears in Collections:CEB - Artigos em Revistas/Séries Internacionais / Papers in International Journals/Series

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