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https://hdl.handle.net/1822/24933
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Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Monteiro, D. R. | - |
dc.contributor.author | Silva, Sónia Carina | - |
dc.contributor.author | Negri, M. | - |
dc.contributor.author | Gorup, L. F. | - |
dc.contributor.author | Camargo, E. R. | - |
dc.contributor.author | Oliveira, Rosário | - |
dc.contributor.author | Barbosa, D. B. | - |
dc.contributor.author | Henriques, Mariana | - |
dc.date.accessioned | 2013-08-05T14:12:15Z | - |
dc.date.available | 2013-08-05T14:12:15Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1364-5072 | por |
dc.identifier.uri | https://hdl.handle.net/1822/24933 | - |
dc.description.abstract | Aim : The aim of this study was to assess the effect of different silver nanoparticles (SN) concentrations on the matrix composition and structure of Candida albicans and Candida glabrata biofilms. Methods and Results : Candida biofilms were developed in 6-well microtiter plates during 48 h. After, these biofilms were exposed to 13·5 or 54 μg SN ml−1 for 24 h. Then, extracellular matrices were extracted from biofilms and analysed chemically in terms of proteins, carbohydrates and DNA. To investigate the biofilm structure, scanning electron microscopy (SEM) and epifluorescence microscopy were used. SN interfered with the matrix composition of Candida biofilms tested in terms of protein, carbohydrate and DNA, except for the protein content of C. albicans biofilm. By SEM, Candida biofilms treated with SN revealed structural differences, when compared with the control groups. Further, SN showed a trend of agglomeration within the biofilms. Epifluorescence microscopy images suggest that SN induced damage on cell walls of the Candida isolates tested. Conclusions : In general, irrespective of concentration, SN affected the matrix composition and structure of Candida biofilms and these findings may be related to the mechanisms of biocide action of SN. Significance and Impact of the Study : This study reveals new insights about the behaviour of SN when in contact with Candida biofilms. SN may contribute to the development of therapies to prevent or control Candida infections. | por |
dc.description.sponsorship | We thank David Williams (Cardiff University, Cardiff, UK) for providing strain 324LA/94. The authors also thank CAPES (grant BEX 1221/10-8) and FAPESP (2009/15146-5), Brazil, for supporting Douglas Roberto Monteiro work. The colloidal suspension of silver nanoparticles used herein was prepared and characterized by LIEC-CMDMC and INCTMN/FAPESP-CNPq, Sao Carlos, Brazil. | por |
dc.language.iso | eng | por |
dc.publisher | Wiley | por |
dc.rights | openAccess | por |
dc.subject | Biofilms | por |
dc.subject | Candida albicans | por |
dc.subject | Candida glabrata | por |
dc.subject | Fungal infections | por |
dc.subject | Silver nanoparticles | por |
dc.title | Silver colloidal nanoparticles : effect on matrix composition and structure of Candida albicans and Candida glabrata biofilms | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 1175 | por |
oaire.citationEndPage | 1183 | por |
oaire.citationIssue | 4 | por |
oaire.citationTitle | Journal of Applied Microbiology | por |
oaire.citationVolume | 114 | por |
dc.publisher.uri | Blackwell Publishing Inc. | por |
dc.identifier.eissn | 1365-2672 | por |
dc.identifier.doi | 10.1111/jam.12102 | - |
dc.identifier.pmid | 23231706 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Applied Microbiology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |