Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/24901

TitleWettability influences cell behavior on superhydrophobic surfaces with different topographies
Author(s)Lourenço, Bianca do Nascimento
Marchioli, Giulia
Welong Song
Reis, R. L.
Blitterswijk, C. A. van
Karperien, Marcel
Apeldoorn, Aart van
Mano, J. F.
KeywordsAttachment
Blood compatibility
Bone
Fibronectin
Interface
Microtopographies
Morphology
Osteoblast adhesion
Poly(l-lactic acid)
Protein adsorption
Issue date2012
PublisherSpringer
JournalBiointerphases
Abstract(s)Surface wettability and topography are recognized as critical factors influencing cell behavior on biomaterials. So far only few works have reported cell responses on surfaces exhibiting extreme wettability in combination with surface topography. The goal of this work is to study whether cell behavior on superhydrophobic surfaces is influenced by surface topography and polymer type. Biomimetic superhydrophobic rough surfaces of polystyrene and poly(l-lactic acid) with different micro/nanotopographies were obtained from smooth surfaces using a simple phase-separation based method. Total protein was quantified and showed a less adsorption of bovine serum albumin onto rough surfaces as compared to smooth surfaces of the same material. The mouse osteoblastic MC3T3-E1 cell line and primary bovine articular chondrocytes were used to study cell attachment and proliferation. Cells attached and proliferate better in the smooth surfaces. The superhydrophobic surfaces allowed cells to adhere but inhibited their proliferation. This study indicates that surface wettability, rather than polymer type or the topography of the superhydrophobic surfaces, is a critical factor in determining cell behavior.
TypeArticle
Description"Published online: 26 July 2012"
URIhttp://hdl.handle.net/1822/24901
DOI10.1007/s13758-012-0046-6
ISSN1934-8630
Publisher versionhttp://dx.doi.org/10.1007/s13758-012-0046-6
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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