Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/20448

TitleImmuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons
Author(s)Pinto, Luísa
Pinheiro, António Mateus
Morais, Mónica
Bessa, J. M.
Sousa, Nuno
KeywordsGolgi-cox
Immunofluorescence
Depression
Stress
Neuroplasticity
Neurogenesis
Issue date2012
PublisherPublic Library of Science
JournalPLoS ONE
Abstract(s)Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this simple noninvasive method, we are able to determine the tridimensional structure of dendritic arborization and spine shape of neurons born at a specific time in the hippocampus of adult animals. This analysis is relevant in physiological and pathological conditions in which altered neurogenesis is implicated, such as aging or emotional disorders
TypeArticle
URIhttp://hdl.handle.net/1822/20448
DOI10.1371/journal.pone.0033114
ISSN1932-6203
Publisher versionhttp://www.plosone.org/
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Artigos em Revistas Internacionais com Referee

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