Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/1945

TitleExtraction of exopolymers from biofilms: the protective effect of glutaraldehyde
Author(s)Azeredo, Joana
Henriques, Mariana
Sillankorva, Sanna
Oliveira, Rosário
KeywordsATP
Biofilm
Cell lysis
Extraction methods
Glutaraldehyde
Issue date2003
PublisherIWA Publishing
International Water Association Publishing
JournalWater Science and Technology
Citation"Water science and technology". ISSN 0273-1223. 47:5 (2003) 175–179.
Abstract(s)The extraction of the exopolymeric matrix is a prerequisite to properly assessing the composition of the biofilm. Several extraction methods have already been developed, however, no universal method has yet been adopted because the compromise between high yields of extraction and minimum cell lysis is difficult to establish. In fact, most of the extraction methods promote leakage of intracellular material. The most common extraction methods, Dowex resin and sonication, were assayed in biofilms of Pseudomonas fluorescens and Alcaligenes denitrificans submitted to a pre-treatment with glutaraldehyde (GTA). The assessment of ATP released after extraction was used as a criterion of cell lysis. The results showed that GTA is a protective agent against cell lysis. The pre-treatment with GTA is particularly useful combined with sonication.
TypeConference paper
URIhttp://hdl.handle.net/1822/1945
ISSN0273-1223
Publisher versionhttp://www.iwaponline.com/wst/04705/wst047050175.htm
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

Files in This Item:
File Description SizeFormat 
2003-03[2].pdf75,5 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID