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https://hdl.handle.net/1822/1945
Título: | Extraction of exopolymers from biofilms: the protective effect of glutaraldehyde |
Autor(es): | Azeredo, Joana Henriques, Mariana Sillankorva, Sanna Oliveira, Rosário |
Palavras-chave: | ATP Biofilm Cell lysis Extraction methods Glutaraldehyde |
Data: | 2003 |
Editora: | International Water Association Publishing (IWAP) |
Revista: | Water Science and Technology |
Citação: | Azeredo, J., Henriques, M., Sillankorva, S., & Oliveira, R. (2003, March 1). Extraction of exopolymers from biofilms: the protective effect of glutaraldehyde. Water Science and Technology. IWA Publishing. http://doi.org/10.2166/wst.2003.0312 |
Resumo(s): | The extraction of the exopolymeric matrix is a prerequisite to properly assessing the composition of the biofilm. Several extraction methods have already been developed, however, no universal method has yet been adopted because the compromise between high yields of extraction and minimum cell lysis is difficult to establish. In fact, most of the extraction methods promote leakage of intracellular material. The most common extraction methods, Dowex resin and sonication, were assayed in biofilms of Pseudomonas fluorescens and Alcaligenes denitrificans submitted to a pre-treatment with glutaraldehyde (GTA). The assessment of ATP released after extraction was used as a criterion of cell lysis. The results showed that GTA is a protective agent against cell lysis. The pre-treatment with GTA is particularly useful combined with sonication. |
Tipo: | Artigo em ata de conferência |
URI: | https://hdl.handle.net/1822/1945 |
DOI: | 10.2166/wst.2003.0312 |
ISSN: | 0273-1223 |
Versão da editora: | http://www.iwaponline.com/wst/04705/wst047050175.htm |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2003-03[2].pdf | 75,5 kB | Adobe PDF | Ver/Abrir |