Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/1945

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dc.contributor.authorAzeredo, Joana-
dc.contributor.authorHenriques, Mariana-
dc.contributor.authorSillankorva, Sanna-
dc.contributor.authorOliveira, Rosário-
dc.date.accessioned2005-06-01T15:30:40Z-
dc.date.available2005-06-01T15:30:40Z-
dc.date.issued2003-
dc.identifier.citation"Water science and technology". ISSN 0273-1223. 47:5 (2003) 175–179.eng
dc.identifier.issn0273-1223-
dc.identifier.urihttp://hdl.handle.net/1822/1945-
dc.description.abstractThe extraction of the exopolymeric matrix is a prerequisite to properly assessing the composition of the biofilm. Several extraction methods have already been developed, however, no universal method has yet been adopted because the compromise between high yields of extraction and minimum cell lysis is difficult to establish. In fact, most of the extraction methods promote leakage of intracellular material. The most common extraction methods, Dowex resin and sonication, were assayed in biofilms of Pseudomonas fluorescens and Alcaligenes denitrificans submitted to a pre-treatment with glutaraldehyde (GTA). The assessment of ATP released after extraction was used as a criterion of cell lysis. The results showed that GTA is a protective agent against cell lysis. The pre-treatment with GTA is particularly useful combined with sonication.eng
dc.description.sponsorshipTraining and mobility of researchers (TMR) - Biological Removal of Nitrogen: from Biofilms to Bioreactors (BiotoBio).por
dc.language.isoengeng
dc.publisherInternational Water Association Publishing (IWAP)-
dc.relation.urihttp://www.iwapublishing.com/template.cfm?name=iwaphome-
dc.rightsopenAccesseng
dc.subjectATPeng
dc.subjectBiofilmeng
dc.subjectCell lysiseng
dc.subjectExtraction methodseng
dc.subjectGlutaraldehydeeng
dc.titleExtraction of exopolymers from biofilms: the protective effect of glutaraldehydeeng
dc.typeconferencePapereng
dc.peerreviewedyeseng
dc.relation.publisherversionhttp://www.iwaponline.com/wst/04705/wst047050175.htm-
oaire.citationStartPage175por
oaire.citationEndPage179por
oaire.citationIssue5por
oaire.citationVolume47por
dc.identifier.pmid12701925-
dc.subject.wosScience & Technologypor
sdum.journalWater Science and Technology-
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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