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dc.contributor.authorPiairo, Paulina C.-
dc.contributor.authorMoura, Rute S.-
dc.contributor.authorSilva, Cristina Nogueira-
dc.contributor.authorPinto, Jorge Correia-
dc.date.accessioned2012-04-18T13:43:29Z-
dc.date.available2012-04-18T13:43:29Z-
dc.date.issued2011-12-
dc.identifier.issn0196-9781por
dc.identifier.urihttps://hdl.handle.net/1822/18736-
dc.description.abstractApelin and its receptor APJ constitute a signaling pathway best recognized as an important regulator of cardiovascular homeostasis. This multifunctional peptidergic system is currently being described to be involved in embryonic events which extend into vascular, ocular and heart development. Additionally, it is highly expressed in pulmonary tissue. Therefore, the aim of this study was to investigate the role of apelinergic system during fetal lung development. Immunohistochemistry and Western blot analysis were used to characterize apelin and APJ expression levels and cellular localization in normal fetal rat lungs, at five different gestational ages as well as in the adult. Fetal rat lung explants were cultured in vitro with increasing doses of apelin. Treated lung explants were morphometrically analyzed and assessed for MAPK signaling modifications. Both components of the apelinergic system are constitutively expressed in the developing lung, with APJ exhibiting monomeric, dimeric and oligomeric forms in the pulmonary tissue. Pulmonary epithelium also displayed constitutive nuclear localization of the receptor. Fetal apelin expression is higher than adult expression. Apelin supplementation inhibitory effect on branching morphogenesis was associated with a dose dependent decrease in p38 and JNK phosphorylation. The results presented provide the first evidence of the presence of an apelinergic system operating in the developing lung. Our findings also suggest that apelin inhibits fetal lung growth by suppressing p38 and JNK signaling pathways.por
dc.description.sponsorshipThis project was funded by Fundação para a Ciência e a Tecnologia (PTDC/SAU-OBD/108051/2008), PP was supported by Fundação para a Ciência e a Tecnologia (reference SFRH/BD/33410/2008). RSM was supported by Fundação para a Ciência e a Tecnologia (reference SFRH/BPD/15408/2005).por
dc.language.isoengpor
dc.publisherElsevier 1por
dc.relationinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/108051/PT-
dc.relationinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/108051/PT-
dc.relationinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F33410%2F2008/PT-
dc.rightsopenAccesspor
dc.subjectApelinpor
dc.subjectAPJpor
dc.subjectLung developmentpor
dc.subjectMAP kinasespor
dc.titleThe apelinergic system in the developing lung: expression and signalingpor
dc.typearticlepor
dc.peerreviewedyespor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0196978111004177por
sdum.publicationstatuspublishedpor
oaire.citationStartPage2474por
oaire.citationEndPage2483por
oaire.citationIssue12por
oaire.citationTitlePeptidespor
oaire.citationVolume32por
dc.identifier.doi10.1016/j.peptides.2011.10.010por
dc.identifier.pmid22015267por
dc.subject.wosScience & Technologypor
sdum.journalPeptidespor
Aparece nas coleções:ICVS - Artigos em revistas internacionais / Papers in international journals

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