Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/17090

TitlePresence of extracellular DNA in candida albicans biofilm matrix and its role in biofilm structure and antifungal susceptibility
Author(s)Martins, Margarida Isabel Barros Coelho
Uppuluri, Priya
Thomas, Derek P.
Cleary, Ian A.
Henriques, Mariana
Lopez-Ribot, José L.
Oliveira, Rosário
Issue dateOct-2010
PublisherUniversidade do Minho. Departamento de Engenharia Biológica (DEB)
Abstract(s)Biofilms are structurally complex microconsortia of surface adhering cells embedded within an extracellular matrix (ECM) composed of substances produced and secreted by cells or derived from cell lysis. One of the recently discovered bacterial biofilms ECM components is the extracellular DNA (eDNA). Although the investigation on eDNA in fungal biofilms is scarce, preliminary studies suggest that eDNA may play a role in biofilms formed by the opportunistic fungal pathogen Candida albicans. Thus, the present study aimed at determining the eDNA content of C. albicans SC5314 biofilm ECM and the effect of DNase I treatment on biofilm formation and biofilm cells susceptibility to antifungals, as indicators of the role of eDNA in C. albicans biofilms. Results from our experiments showed that the ECM of C. albicans biofilms formed under conditions of flow for 48 h contained 3045.4 ± 227.3 ng eDNA/mg of protein. Additionally, using a microtiter plate model, we observed that different DNase treatments (0.02 - 2 mg/ml) did not affect further biofilm development by C. albicans adherent cells. However, DNase (> 0.03 mg/ml) promoted a general biomass reduction on C. albicans preformed biofilms. Finally, DNase (0.13 mg/ml) did not change C. albicans biofilm cells susceptibility to fluconazole, but increased their susceptibility to amphotericin B and caspofungin, as indicated by the lower SMIC compared to biofilms grown without DNase. This work presents evidence for the role of eDNA in C. albicans biofilm integrity and antifungal resistance consistent with eDNA being a key element of the ECM.
TypeAbstract
URIhttp://hdl.handle.net/1822/17090
ISBN978-972-97810-6-3
Publisher versionhttp://www.ceb.uminho.pt/2nd_Meeting_IBB/Downloads/2ndMeetingIBB_Book_of_Abstracts.pdf
Peer-Reviewedno
AccessOpen access
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

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