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TitleMonoolein as helper lipid for non-viral transfection in mammals
Author(s)Oliveira, Ana Cristina Norberto Gonçalves
Silva, J. P. Neves
Coutinho, Paulo J. G.
Gomes, A. A.
Coutinho, O. P.
Oliveira, M. Elisabete
Issue date2010
PublisherElsevier Science BV
JournalJournal of Controlled Release
Abstract(s)Lipoplexes composed of pDNA and DODAB/MO at different molar ratios (4:1, 2:1 and 1:1) and different cationic lipid/DNA charge ratios were investigated. The physicochemical properties of the lipoplexes (size and charge), the pDNA complexation, and the effect of heparin on pDNA release, were studied by Dynamic Light Scattering, Zeta Potential, and Ethidium Bromide exclusion assays. The cytotoxicity, transfection efficiency and the intracellular localization of DNA were evaluated on 293T cells.
AccessOpen access
Appears in Collections:CDF - FAMO - Comunicações/Communications (with refereeing)
DBio - Comunicações/Communications in Congresses

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