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https://hdl.handle.net/1822/91239
Título: | Oregano essential oil: an effective and non-toxic approach for prevent or treat resistant candida species |
Autor(es): | Fernandes, Liliana Sousa Silva, Inês Blázquez, Elena Tejada, Ainara Ribeiro, Artur Silva, Sónia Carina Mira, Nuno Cussó, Lorena Costa-de-Oliveira, Sofia Rodrigues, Maria Elisa Costa Henriques, Mariana |
Palavras-chave: | Vulvovaginal candidiasis Lactobacillus spp Phytotherapeutic applications Vapor phase of essential oil Keratin nanocapsules Alternative treatment |
Data: | 7-Dez-2023 |
Editora: | Universidade da Beira Interior (UBI) |
Citação: | Fernandes, L.; Silva, Inês; Blázquez, Elena; Tejada, Ainara; Ribeiro, Artur; Silva, Sónia Carina; Mira, Nuno; Cussó, Lorena; Costa-de-Oliveira, Sofia; Rodrigues, M. Elisa; Henriques, Mariana, Oregano essential oil: an effective and non-toxic approach for prevent or treat resistant candida species. Microbiotec23 - Congress of Microbiology and Biotechnology 2023. No. P3.6, Covilhã, Portugal, Dec 07-09, 325-326, 2023. |
Resumo(s): | Vulvovaginal candidiasis (VVC) is one of the most prevalent vaginal infectious diseases, and the emergence of drug-resistant Candida strains has presented a growing challenge in its treatment. This highlights the urgent need to develop effective and non-toxic alternative treatments. In this context, essential oils (EOs) have emerged as a promising alternative considering low toxicity and high antimicrobial activity. This work is divided into two parts, the first consists of evaluating the effect of the vapor phase of oregano EO (VP-OEO) on biofilms of antifungal-resistant Candida species (Candida albicans and Candida glabrata) quantified by colony forming units enumeration and determine their mode of action by flow cytometry. Interestingly, the VP-EOs has shown to be more effective against Candida growth than their liquid form. Indeed, the results revealed high antifungal activity of VP-OEO against these drug-resistant strains, significantly reducing biofilm formation and mature biofilms, with impact on membrane integrity and metabolic activity of the fungal cells. The second part consists of the design and evaluation of nanoencapsulated OEO (KNP-OEO) as another alternative application of OEO for VVC treatment. These nanoparticles provided stability to OEO and controlled release of the EO. The results demonstrated complete inhibition of C. albicans growth. Moreover, in in vivo assay with BALB/C female mice, a single intravaginal application of KNP-OEO reduced C. albicans growth and preserved a healthy vaginal microbiota, including Lactobacillus species. In conclusion, these studies highlight the promising efficacy of OEO as an alternative for VVC treatment. Both approaches, VP-OEO and OEO-KNP, showed effective antifungal activity against drug-resistant strains while preserving vaginal health. These therapeutic options not only combat antifungal resistance, but also potentially propose a safer option for women's health due to their natural characteristics. However, further research is needed to confirm these promising results and advance the development of these alternative VVC therapies. |
Tipo: | Resumo em ata de conferência |
URI: | https://hdl.handle.net/1822/91239 |
Versão da editora: | https://microbiotec23.organideia.com/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
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Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_57708_1.pdf | 246,98 kB | Adobe PDF | Ver/Abrir |