Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/90543
Título: | Preparation of a fluorescent peptide substrate to target tumor-associated macrophages |
Autor(es): | Leite, Ângela B. M. P. Martins, Cátia D. F. Raposo, M. Manuela M. Costa, Susana P. G. |
Palavras-chave: | Fluorescent substrates M2 macrophages Peptide labeling Rhodamine B Tumor targeting |
Data: | 2023 |
Editora: | MDPI |
Citação: | Leite, A. B. M. P.; Martins, C. D.; Raposo, M. M. M.; Costa, S. P. G. Preparation of a Fluorescent Peptide Substrate to Target Tumor-associated Macrophages. Proceedings of the 27th International Electronic Conference on Synthetic Organic Chemistry. Chem. Proc. 2023, 14(1), 63. |
Resumo(s): | One of the significant challenges in targeting M2 macrophages is the need for specific and high-affinity targeting ligands that can distinguish them from phenotype M1 macrophages. In order to investigate the selectivity of the polypeptide M2pep (YEQDPWGVKWWY) towards M2 macrophages using fluorescence-based techniques, we report here the synthesis and characteriza-tion of the M2pep substrate labeled with a fluorophore. This peptide comprising a 12-amino acid sequence with a spacer consisting of three additional glycines (GGG) was synthesized and cou-pled to Rhodamine B at its N-terminal via microwave-assisted solid-phase synthesis. The structure of the labeled peptide was confirmed by NMR, UV-Vis, fluorescence spectroscopy and mass spec-trometry. |
Tipo: | Artigo em ata de conferência |
URI: | https://hdl.handle.net/1822/90543 |
DOI: | 10.3390/ecsoc-27-16101 |
ISSN: | 2673-4583 |
Versão da editora: | https://doi.org/10.3390/ecsoc-27-16101 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CDQuim - Comunicações e Proceedings |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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chemproc-14-00063.pdf | proceeding | 887,7 kB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons