Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/88771
Título: | Contactless resolution of inflammatory signals in tailored macrophage-based cell therapeutics |
Autor(es): | Almeida, Ana F. Miranda, Margarida Silva Vinhas, Carla Adriana Araújo Rodrigues, Márcia T. Gomes, Manuela E. |
Palavras-chave: | Macrophages Magnetoplexes miR-155 RNA delivery SPION |
Data: | Ago-2023 |
Editora: | American Chemical Society (ACS) |
Revista: | ACS Applied Materials and Interfaces |
Citação: | Almeida A. F., Miranda M. S., Vinhas A., Rodrigues M. T., Gomes M. E. Contactless Resolution of Inflammatory Signals in Tailored Macrophage-Based Cell Therapeutics, ACS Appl Mater Interfaces , Vol. 15, Issue 44, pp. 50612–50625, doi:10.1021/acsami.2c22505., 2023 |
Resumo(s): | In recent years, nanotechnology-based microRNA (miR) therapeutic platforms have shown great promise for immunotherapy and tissue regeneration, despite the unmet challenge of achieving efficient and safe delivery of miRs. The transport of miRs offers precision and regulatory value for a myriad of biological processes and pathways, including the control of macrophage (MÏ ) functions and, consequently, the inflammatory cascades MÏ are involved in. Thus, enforcement of MÏ can boost the regenerative process and provide new solutions for diverse chronic pathologies. In this study, we sought to develop a magnetically guided transporter to deliver an miR-155 antagonist to M1-primed MÏ . Furthermore, we determined its modulatory effect in reprogramming MÏ from inflammatory to pro-regenerative phenotypes, with the aim of tissue healing and regenerative medicine approaches. This strategy combines contactless and high-precision control of MÏ , anticipating new functional miR carriers for targeted strategies controlled by extracorporeal action. The magnetoplexes SPION@PEI-miR were efficiently delivered into MÏ without compromising cell viability and successfully induced miR-mediated gene silencing by enhancing the expression of anti-inflammatory markers (IL4 and IL10) and the production of M2Ï -related markers (CD206 and IL4). Given its multimodal features, SPION@PEI-miR represents a simple, safe, and nonviral theranostic platform that enables imaging, tracking, and miR delivery with modulatory effects on immune cells. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/88771 |
DOI: | 10.1021/acsami.2c22505. |
ISSN: | 1944-8244 |
Versão da editora: | https://doi.org/10.1021/acsami.2c22505 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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21035-ana-f-almeida-et-al-2023-paper-2-acs-matint.pdf | 11,38 MB | Adobe PDF | Ver/Abrir |