Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/88570
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Rodrigues, Daniel Barreira | por |
dc.contributor.author | Moreira, Helena R. | por |
dc.contributor.author | Cerqueira, Mariana T. | por |
dc.contributor.author | Marques, A. P. | por |
dc.contributor.author | Castro, António G. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Pirraco, Rogério P. | por |
dc.date.accessioned | 2024-02-06T12:38:06Z | - |
dc.date.available | 2024-02-06T12:38:06Z | - |
dc.date.issued | 2022-09 | - |
dc.identifier.citation | Rodrigues D. B., Moreira H. R., Cerqueira M. T., Marques A. P., Castro A. G., Reis R. L., Pirraco R. P. Highly tailorable gellan gum nanoparticles as a platform for the development of T cell activator systems, Biomaterials Research, Vol. 26, pp. 48, doi:10.1186/s40824-022-00297-z, 2022 | por |
dc.identifier.issn | 1226-4601 | por |
dc.identifier.uri | https://hdl.handle.net/1822/88570 | - |
dc.description.abstract | Background: T cell priming has been shown to be a powerful immunotherapeutic approach for cancer treatment in terms of efcacy and relatively weak side efects. Systems that optimize the stimulation of T cells to improve therapeutic efcacy are therefore in constant demand. A way to achieve this is through artifcial antigen presenting cells that are complexes between vehicles and key molecules that target relevant T cell subpopulations, eliciting antigenspecifc T cell priming. In such T cell activator systems, the vehicles chosen to deliver and present the key molecules to the targeted cell populations are of extreme importance. In this work, a new platform for the creation of T cell activator systems based on highly tailorable nanoparticles made from the natural polymer gellan gum (GG) was developed and validated. Methods: GG nanoparticles were produced by a water in oil emulsion procedure, and characterized by dynamic light scattering, high resolution scanning electronic microscopy and water uptake. Their biocompatibility with cultured cells was assessed by a metabolic activity assay. Surface functionalization was performed with anti-CD3/ CD28 antibodies via EDC/NHS or NeutrAvidin/Biotin linkage. Functionalized particles were tested for their capacity to stimulate CD4+ T cells and trigger T cell cytotoxic responses. Results: Nanoparticles were approximately 150 nm in size, with a stable structure and no detectable cytotoxicity. Water uptake originated a weight gain of up to 3200%. The functional antibodies did efciently bind to the nanoparticles, as confrmed by SDS-PAGE, which then targeted the desired CD4+ populations, as confrmed by confocal microscopy. The developed system presented a more sustained T cell activation over time when compared to commercial alternatives. Concurrently, the expression of higher levels of key cytotoxic pathway molecules granzyme B/perforin was induced, suggesting a greater cytotoxic potential for future application in adoptive cancer therapy. Conclusions: Our results show that GG nanoparticles were successfully used as a highly tailorable T cell activator system platform capable of T cell expansion and re-education. | por |
dc.description.sponsorship | Authors would like to acknowledge the fnancial support from the European Research Council through the Starting Grant “CapBed” (ERC-2018-STG-805411), FCT/MCTES (Fundação para a Ciência e a Tecnologia/ Ministério da Ciência, Tecnologia, e Ensino Superior) through the grants SFRH/BD/119756/2016 (D.B.R.) and IF/00347/2015 (R.P.P.) and to the FSE/POCH (Fundo Social Europeu através do Programa Operacional do Capital Humano) under the scope of the PD/169/2013, NORTE-08–5369-FSE-000037 (H.R.M.). | por |
dc.language.iso | eng | por |
dc.publisher | BioMed Central (BMC) | por |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/805411/EU | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F119756%2F2016/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00347%2F2015%2FCP1294%2FCT0004/PT | por |
dc.rights | openAccess | por |
dc.subject | Cytotoxic T cells | por |
dc.subject | Gellan Gum | por |
dc.subject | Nanoparticles | por |
dc.subject | T cell stimulation | por |
dc.title | Highly tailorable gellan gum nanoparticles as a platform for the development of T cell activator systems | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://dx.doi.org/10.1186/s40824-022-00297-z | por |
dc.comments | http://3bs.uminho.pt/node/20917 | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 26 | por |
dc.date.updated | 2024-02-06T09:27:01Z | - |
dc.identifier.eissn | 2055-7124 | por |
dc.identifier.doi | 10.1186/s40824-022-00297-z | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biomaterials Research | por |
dc.identifier.articlenumber | 48 | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
20917-rodrigues-et-al-2022.pdf | 7,21 MB | Adobe PDF | Ver/Abrir |