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https://hdl.handle.net/1822/88269
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Campo DC | Valor | Idioma |
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dc.contributor.author | Baptista, Marlene Isabel Cerqueira Montenegro | por |
dc.contributor.author | Costa, Carlos E. | por |
dc.contributor.author | Domingues, Lucília | por |
dc.date.accessioned | 2024-01-24T08:48:01Z | - |
dc.date.available | 2024-01-24T08:48:01Z | - |
dc.date.issued | 2023-09-07 | - |
dc.identifier.citation | Baptista, Marlene; Costa, Carlos E.; Domingues, Lucília, Microbial Production of the building block p-Coumaric Acid: Unleashing the Potential of Kluyveromyces marxianus. Microbiotec23 - Congress of Microbiology and Biotechnology 2023. No. P5.15, Covilhã, Portugal, Dec 07-09, 497, 2023. | por |
dc.identifier.uri | https://hdl.handle.net/1822/88269 | - |
dc.description.abstract | The interest in several plant secondary metabolites, such as naringenin or resveratrol, for their known biological functions, is growing at a fast pace. These properties encompass antioxidant, antiinflammatory, and anti-microbial, among many other activities. The phenolic acid p-coumaric acid is naturally produced by plants, being a key precursor of many of these secondary metabolites. Nevertheless, p-coumaric acid is currently extracted from plants, which poses several drawbacks for its industrial production including low efficiency and dependence on plant availability. On the other hand, microbial production of p-coumaric acid has been emerging as a sustainable and economically viable alternative. The unconventional yeast Kluyveromyces marxianus is garnering increasing interest as an alternative cell platform to produce ethanol and high-value compounds with a span of applications across industries [1]. This is due to its distinctive attributes, such as rapid growth rate, thermotolerance, and the capacity to metabolize different sugars [2]. Due to its Crabtree-negative metabolism, this yeast produces acetyl-coenzyme A in the presence of oxygen and high sugar concentration. As such, it could be an interesting chassis to produce aromatic compounds derived from p-coumaric since some of them require malonyl-CoA (derived from acetyl-CoA) as a precursor. For that, in yeast, the expression of heterologous enzymes involved in the conversion of aromatic amino acids into p-coumaric acid is required. Building upon this knowledge, here, two K. marxianus strains were engineered and screened for their capacity for p-coumaric acid production. Initially, a heterologous enzyme, tyrosine ammonia lyase (TAL), which converts tyrosine to p-coumaric acid, was integrated into both strains. Further, the effects of different carbon sources and agitation conditions on p-coumaric acid production and yeast primary metabolism were evaluated. Overall, this work shows the potential of K. marxianus for p-coumaric acid production and its derivatives through an integrated process. | por |
dc.description.sponsorship | Portuguese Foundation for Science and Technology (FCT) - strategic funding of UIDB/ 04469/2020; Marlene Baptista PhD scholarship (2020.06888.BD) | por |
dc.language.iso | eng | por |
dc.publisher | Universidade da Beira Interior (UBI) | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/2020.06888.BD/PT | por |
dc.rights | openAccess | por |
dc.subject | Kluyveromyces marxianus | por |
dc.subject | p-coumaric acid | por |
dc.subject | Tyrosine ammonia lyase | por |
dc.subject | Precision fermentation | por |
dc.title | Microbial production of the building block p-Coumaric Acid: unleashing the potential of Kluyveromyces marxianus | por |
dc.type | conferenceAbstract | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://microbiotec23.organideia.com/ | por |
dc.comments | CEB56577 | por |
sdum.event.type | conference | por |
oaire.citationStartPage | 497 | por |
oaire.citationIssue | P5.15 | - |
oaire.citationConferencePlace | Covilhã, Portugal | por |
dc.date.updated | 2024-01-23T20:51:36Z | - |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
sdum.conferencePublication | Microbiotec23 - Congress of Microbiology and Biotechnology 2023 | por |
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