Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/85094
Título: | Levofloxacin-loaded microneedles produced using 3D-printed molds for Klebsiella pneumoniae biofilm control |
Autor(es): | Vinayakumar, K. B. Silva, Maria Daniela Martins, Artur Mundy, Stephen González-Losada, Pedro Sillankorva, Sanna M. |
Palavras-chave: | 3D printing biofilms Klebsiella pneumoniae levofloxacin microneedles polyvinyl alcohol |
Data: | Jun-2023 |
Editora: | Wiley |
Revista: | Advanced Therapeutics |
Citação: | Vinayakumar, K. B.; Silva, Maria Daniela; Martins, Artur; Mundy, Stephen; González-Losada, Pedro; Sillankorva, Sanna M., Levofloxacin-loaded microneedles produced using 3D-printed molds for Klebsiella pneumoniae biofilm control. Advanced Therapeutics, 6(6), 2200320, 2023 |
Resumo(s): | Additive manufacturing advancements contribute considerably to several fields, and its use in the medical field is gaining attention due to its easily customizable option (patient-specific), low cost, and fast turnout time in developing drug delivery and diagnostic tools. Here, wereport the fabrication of a microneedle (MN) platform using a stereolithography 3D printer, varying the 3D printing angle and aspect ratio (2:1, 3:1, 4:1). The optimal printing angle was30, resulting in needle tip and base diameters of 50 µm and 330 µm, and heights of 550/850/1180 µm. Polyvinyl alcohol (PVA) MNs produced with varying levofloxacin concentrations showed variability of 4% in tip and 3% base diameters and 15% in height compared to the 3D-printed MNs. Geometry B wasused to produce levofloxacin-loaded PVA MNs and tested against Klebsiella pneumoniae colony biofilms. Levofloxacin wasreleased gradually, as assessed by spectrofluorimetry. The MIC of levofloxacin against the K. pneumoniae clinical isolate was4 µg/mL, but this concentration wasinsufficient to cause any effect on K. pneumoniae biofilms. Only concentrations 32 µg/mL werestatistically different compared to the unloaded MNs. 3D printing is an attractive solution to produce molds for fabricating biopolymeric MNs for topical drug delivery. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/85094 |
DOI: | 10.1002/adtp.202200320 |
ISSN: | 2366-3987 |
Versão da editora: | https://onlinelibrary.wiley.com/doi/10.1002/adtp.202200320 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
document_56159_1.pdf | 2,94 MB | Adobe PDF | Ver/Abrir |