Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/85094

TítuloLevofloxacin-loaded microneedles produced using 3D-printed molds for Klebsiella pneumoniae biofilm control
Autor(es)Vinayakumar, K. B.
Silva, Maria Daniela
Martins, Artur
Mundy, Stephen
González-Losada, Pedro
Sillankorva, Sanna M.
Palavras-chave3D printing
biofilms
Klebsiella pneumoniae
levofloxacin
microneedles
polyvinyl alcohol
DataJun-2023
EditoraWiley
RevistaAdvanced Therapeutics
CitaçãoVinayakumar, K. B.; Silva, Maria Daniela; Martins, Artur; Mundy, Stephen; González-Losada, Pedro; Sillankorva, Sanna M., Levofloxacin-loaded microneedles produced using 3D-printed molds for Klebsiella pneumoniae biofilm control. Advanced Therapeutics, 6(6), 2200320, 2023
Resumo(s)Additive manufacturing advancements contribute considerably to several fields, and its use in the medical field is gaining attention due to its easily customizable option (patient-specific), low cost, and fast turnout time in developing drug delivery and diagnostic tools. Here, wereport the fabrication of a microneedle (MN) platform using a stereolithography 3D printer, varying the 3D printing angle and aspect ratio (2:1, 3:1, 4:1). The optimal printing angle was30, resulting in needle tip and base diameters of 50 µm and 330 µm, and heights of 550/850/1180 µm. Polyvinyl alcohol (PVA) MNs produced with varying levofloxacin concentrations showed variability of 4% in tip and 3% base diameters and 15% in height compared to the 3D-printed MNs. Geometry B wasused to produce levofloxacin-loaded PVA MNs and tested against Klebsiella pneumoniae colony biofilms. Levofloxacin wasreleased gradually, as assessed by spectrofluorimetry. The MIC of levofloxacin against the K. pneumoniae clinical isolate was4 µg/mL, but this concentration wasinsufficient to cause any effect on K. pneumoniae biofilms. Only concentrations 32 µg/mL werestatistically different compared to the unloaded MNs. 3D printing is an attractive solution to produce molds for fabricating biopolymeric MNs for topical drug delivery.
TipoArtigo
URIhttps://hdl.handle.net/1822/85094
DOI10.1002/adtp.202200320
ISSN2366-3987
Versão da editorahttps://onlinelibrary.wiley.com/doi/10.1002/adtp.202200320
Arbitragem científicayes
AcessoAcesso aberto
Aparece nas coleções:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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