Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/78771
Título: | Influence of PDLA nanoparticles size on drug release and interaction with cells |
Autor(es): | Cartaxo, Ana Luísa Costa-Pinto, Ana R. Martins, Albino Faria, Susana Gonçalves, Virgínia M. F. Tiritan, Maria Elizabeth Ferreira, Helena Susana Costa Machado Neves, N. M. |
Palavras-chave: | Cell internalization Cytocompatibility PDLA nanoparticles Prednisolone Size distribution |
Data: | 2019 |
Editora: | Wiley |
Revista: | Journal of Biomedical Materials Research - Part A |
Citação: | Cartaxo, A. L., Costa-Pinto, A. R., Martins, A., Faria, S., Gonçalves, V. M. F., Tiritan, M. E., … Neves, N. M. (2018, November 28). Influence of PDLA nanoparticles size on drug release and interaction with cells. Journal of Biomedical Materials Research Part A. Wiley. http://doi.org/10.1002/jbm.a.36563 |
Resumo(s): | Polymeric nanoparticles (NPs) are strong candidates for the development of systemic and targeted drug delivery applications. Their size is a determinant property since it defines the NP-cell interactions, drug loading capacity, and release kinetics. Herein, poly(d,l-lactic acid) (PDLA) NPs were produced by the nanoprecipitation method, in which the influence of type and concentration of surfactant as well as PDLA concentration were assessed. The adjustment of these parameters allowed the successful production of NPs with defined medium sizes, ranging from 80 to 460 nm. The surface charge of the different NPs populations was consistently negative. Prednisolone was effectively entrapped and released from NPs with statistically different medium sizes (i.e., 80 or 120 nm). Release profiles indicate that these systems were able to deliver appropriate amounts of drug with potential applicability in the treatment of inflammatory conditions. Both NPs populations were cytocompatible with human endothelial and fibroblastic cells, in the range of concentrations tested (0.187-0.784 mg/mL). However, confocal microscopy revealed that within the range of sizes tested in our experiments, NPs presenting a medium size of 120 nm were able to be internalized in endothelial cells. In summary, this study demonstrates the optimization of the processing conditions to obtain PDLA NPs with narrow size ranges, and with promising performance for the treatment of inflammatory diseases. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/78771 |
DOI: | 10.1002/jbm.a.36563 |
ISSN: | 1552-4965 |
Versão da editora: | https://onlinelibrary.wiley.com/doi/full/10.1002/jbm.a.36563 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19684-Influence of PDLA nanoparticles size on drug release and interaction with.pdf | 948,05 kB | Adobe PDF | Ver/Abrir |