Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/69425
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Domínguez-Arca, V. | por |
dc.contributor.author | Costa, R. R. | por |
dc.contributor.author | Carvalho, Ana Margarida Moura Pereira Ferreira | por |
dc.contributor.author | Taboada, P. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Prieto, G. | por |
dc.contributor.author | Pashkuleva, I. | por |
dc.date.accessioned | 2021-01-18T16:02:06Z | - |
dc.date.issued | 2021 | - |
dc.date.submitted | 2020-09 | - |
dc.identifier.citation | Domínguez-Arca, V., Costa, R. R., Carvalho, A. M., Taboada, P., Leis, R. R., Prieto, G., & Pashkuleva, I. (2021). Liposomes embedded in layer by layer constructs as simplistic extracellular vesicles transfer model. Materials Science and Engineering: C, 111813 | por |
dc.identifier.issn | 0928-4931 | por |
dc.identifier.uri | https://hdl.handle.net/1822/69425 | - |
dc.description | Journal Pre-proof | por |
dc.description.abstract | Extracellular vesicles (EVs) are particles originating from the exfoliation of the cellular membrane. They are involved in cell-to-cell and cell-to-matrix signaling, exchange of bioactive molecules, tumorigenesis and metastasis, among others. To mitigate the limited understanding of EVs transfer phenomena, we developed a simplistic model that mimics EVs and their interactions with cells and the extracellular matrix. The proposed model is a layer-by-layer (LbL) film built from the polycationic poly-L-lysine (PLL) and the glycosaminoglycan hyaluronic acid (HA) to provide ECM mimicry. Positively charged 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and N1,N1,N14,N14-tetramethyl-N1,N14-ditetradecyltetradecane-1,14-diaminium dibromide (GS14) liposomes were embedded in this construct to act as EVs analogs. To simulate EVs carrying substances, Nile Red was loaded as a model of lipophilic cargo molecules. The integration of each component was followed by quartz-crystal microbalance measurements, which confirmed the immobilization of intact liposomes on the underlying (PLL/HA)3 soft film. The release of Nile Red from liposomes either embedded in the LbL construct or exposed at its surface revealed a fast first order release. This system was validated as a model for EV/cell interactions by incubation with breast cancer cells MDA-MB-231. We observed higher internalization for embedded liposomes when compared with surface-exposed ones, showcasing that the ECM mimic layers do not constitute a barrier to liposome/cell interactions but favor them. | por |
dc.description.sponsorship | Programa Iacobus, the financial support from the Portuguese Fundação para a Ciência e a Tecnologia (project PTDC/BTM-MAT/28327/2017, and grants CEECIND/02842/2017, SFRH/BD/114847/2016), the European Commission through the Horizon 2020 framework (ELASTISLET NMP-2014-646075 and FORECAST 668983), the Spanish AEI and the European Regional Development Fund (MAT2016-80266-R). Facilities provided by the Galician Supercomputing Centre (CESGA) are also acknowledged. This work was also supported by Ministerio de Economia y Competitividad of Spain (project MAT2016-80266-R), Xunta de Galicia (Grupo de Referencia Competitiva ED431C 2018/26; Agrupación Estratégica de Materiales-AEMAT ED431E 2018/08). The European Union (through European Regional Development Funds-ERDF) | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.rights | restrictedAccess | por |
dc.subject | Cell communication | por |
dc.subject | Extracellular matrix | por |
dc.subject | Glycosaminoglycans | por |
dc.subject | Layer-by-layer | por |
dc.subject | Liposomes | por |
dc.subject | Supported vesicle layers | por |
dc.title | Liposomes embedded in layer by layer constructs as simplistic extracellular vesicles transfer model | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0928493120337322 | por |
dc.comments | http://3bs.uminho.pt/node/20438 | por |
oaire.citationVolume | 121 | por |
dc.date.updated | 2021-01-04T15:45:08Z | - |
dc.identifier.doi | 10.1016/j.msec.2020.111813 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.pmid | 33579457 | por |
dc.subject.fos | Engenharia e Tecnologia::Biotecnologia Industrial | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Materials Science and Engineering: C | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20438-1-s2.0-S0928493120337322-main.pdf Acesso restrito! | 1,39 MB | Adobe PDF | Ver/Abrir |