Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/66421
Título: | Magnetic stimulation drives macrophage polarization in cell to–cell communication with IL-1β primed tendon cells |
Autor(es): | Vinhas, Adriana Almeida, Ana Filipa Martins Gonçalves, A I Rodrigues, Márcia T. Gomes, Manuela E. |
Palavras-chave: | co-cultures magnetic stimuli macrophages human tendon cells IL-1 beta cell communication inflammation tendon regenerative medicine IL-1β |
Data: | 30-Jul-2020 |
Editora: | Multidisciplinary Digital Publishing Institute |
Revista: | International Journal of Molecular Sciences |
Citação: | Vinhas, A.; Almeida, A.F.; Gonçalves, A.I.; Rodrigues, M.T.; Gomes, M.E. Magnetic Stimulation Drives Macrophage Polarization in Cell to–Cell Communication with IL-1β Primed Tendon Cells. Int. J. Mol. Sci. 2020, 21, 5441. |
Resumo(s): | Inflammation is part of the natural healing response, but it has been simultaneously associated with tendon disorders, as persistent inflammatory events contribute to physiological changes that compromise tendon functions. The cellular interactions within a niche are extremely important for healing. While human tendon cells (hTDCs) are responsible for the maintenance of tendon matrix and turnover, macrophages regulate healing switching their functional phenotype to environmental stimuli. Thus, insights on the hTDCs and macrophages interactions can provide fundamental contributions on tendon repair mechanisms and on the inflammatory inputs in tendon disorders. We explored the crosstalk between macrophages and hTDCs using co-culture approaches in which hTDCs were previously stimulated with IL-1β. The potential modulatory effect of the pulsed electromagnetic field (PEMF) in macrophage-hTDCs communication was also investigated using the magnetic parameters identified in a previous work. The PEMF influences a macrophage pro-regenerative phenotype and favors the synthesis of anti-inflammatory mediators. These outcomes observed in cell contact co-cultures may be mediated by FAK signaling. The impact of the PEMF overcomes the effect of IL-1β-treated-hTDCs, supporting PEMF immunomodulatory actions on macrophages. This work highlights the relevance of intercellular communication in tendon healing and the beneficial role of the PEMF in guiding inflammatory responses toward regenerative strategies. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/66421 |
DOI: | 10.3390/ijms21155441 |
ISSN: | 1661-6596 |
e-ISSN: | 1422-0067 |
Versão da editora: | https://www.mdpi.com/1422-0067/21/15/5441 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
ijms-21-05441.pdf | 3,29 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons