Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/62872
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Costa-Almeida, R. | por |
dc.contributor.author | Calejo, I. | por |
dc.contributor.author | Altieri, R. | por |
dc.contributor.author | Domingues, Rui Miguel Andrade | por |
dc.contributor.author | Giordano, E. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Gomes, Manuela E. | por |
dc.date.accessioned | 2020-01-06T09:39:20Z | - |
dc.date.available | 2020-04-01T06:00:20Z | - |
dc.date.issued | 2019-03 | - |
dc.date.submitted | 2019-03 | - |
dc.identifier.citation | Costa-Almeida R., Calejo I., Altieri R., Domingues R. M. A., Giordano E., Reis R. L., Gomes M. E. Exploring platelet lysate hydrogel-coated suture threads as biofunctional composite living fibers for cell delivery in tissue repair, Biomedical Materials, doi:10.1088/1748-605X/ab0de6, 2019 | por |
dc.identifier.issn | 1748-605X | por |
dc.identifier.uri | https://hdl.handle.net/1822/62872 | - |
dc.description.abstract | To engineer functional tissue substitutes, it is required a multi-component, multi-scale approach that combines both physical, chemical and biological cues. Fiber-based techniques have been explored in the field of tissue engineering to produce structures recapitulating tissue architecture and mechanical properties. In this work, we engineered biofunctional composite living fibers (CLF) as multi-compartment fibers with a mechanically competent core and a hydrogel layer. For this purpose, commercial silk suture threads were coated with a platelet lysate (PL) hydrogel by first embedding the threads in a thrombin solution and then incubating in PL. The fabrication set-up was optimized and the biological performance was studied by encapsulating human adipose-derived stem cells (hASCs). The developed coating process rendered CLF with a homogenous PL hydrogel layer covering suture threads. Encapsulated hASCs were viable up to 14 days in culture and were able to align at the surface of the core fiber and deposit collagen types I and III. In summary, the study shows that PL-hASCs hydrogel coated suture threads represent a simple multi-compartment and multifunctional system, with PL hydrogel offering biofunctionality to guide the biological activities of encapsulated cells in addition to the replication of tissue-level mechanical support provided by the suture threads. | por |
dc.description.sponsorship | Work developed under the framework of the Cooperation Agreement established with the Serviço de Imuno-Hemoterapia do Centro Hospitalar de S. João, EPE. The authors would like to thank to the Plastic Surgery Department of Hospital da Prelada (Porto, Portugal) for providing lipoaspirate tissue samples. Authors acknowledge the financial support from the ERC Grant CoG MagTendon nr 772817; and the Project NORTE-01-0145-FEDER-000021 (RCA pos-doc grant): “Accelerating tissue engineering and personalized medicine discoveries by the integration of key enabling nanotechnologies, marine-derived biomaterials and stem cells”, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF); FCT–Fundação para a Ciência e a Tecnologia for the PhD grant of IC (PD/BD/128088/2016) and pos-doc grant of RMAD (SFRH/BPD/112459/2015); and HORIZON 2020 under the TEAMING Grant agreement No 739572 - The Discoveries CTR. R.A. was the recipient of a scholarship from the School of Engineering of the University of Bologna (Italy). | por |
dc.language.iso | eng | por |
dc.publisher | IOP Publishing | por |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/772817/EU | por |
dc.relation | info:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBPD%2F112459%2F2015/PT | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POR_NORTE/PD%2FBD%2F128088%2F2016/PT | por |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/739572/EU | por |
dc.rights | openAccess | por |
dc.subject | Biofunctional composite living sutures | por |
dc.subject | cell encapsulation | por |
dc.subject | Cell-laden fibers | por |
dc.subject | fiber-based techniques | por |
dc.subject | Multi-compartment fibers | por |
dc.subject | Tissue engineering | por |
dc.title | Exploring platelet lysate hydrogel-coated suture threads as biofunctional composite living fibers for cell delivery in tissue repair | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://doi.org/10.1088/1748-605X/ab0de6 | por |
dc.comments | http://3bs.uminho.pt/node/19805 | por |
oaire.citationIssue | 3 | por |
oaire.citationVolume | 14 | por |
dc.date.updated | 2020-01-03T19:46:12Z | - |
dc.identifier.doi | 10.1088/1748-605X/ab0de6 | por |
dc.identifier.pmid | 30844766 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biomedical Materials | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19805-Costa-Almeida et al 2019_PL biofunctional CLF_Biomedical Materials.pdf | Accepted Manuscript | 1,58 MB | Adobe PDF | Ver/Abrir |