Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/60737
Título: | A textile platform using continuous aligned and textured composite microfibers to engineer tendon-to-bone interface gradient scaffolds |
Autor(es): | Calejo, Isabel Almeida, Raquel Carvalho Ferreira Costa Reis, R. L. Gomes, Manuela E. |
Palavras-chave: | Biotextiles Cell-laden microfibers Gradient Biomaterials Tendon-to-bone interfaces Wet spinning |
Data: | 2019 |
Editora: | Wiley |
Revista: | Advanced Healthcare Materials |
Citação: | Calejo I., Costa-Almeida R., Reis R. L., Gomes M. E. A Textile Platform Using Continuous Aligned and Textured Composite Microfibers to Engineer Tendon-to-Bone Interface Gradient Scaffolds, Advanced Healthcare Materials, doi:10.1002/adhm.201900200, 2019 |
Resumo(s): | Tendon-to-bone interfaces exhibit a hierarchical multitissue transition. To replicate the progression from mineralized to nonmineralized tissue, a novel 3D fibrous scaffold is fabricated with spatial control over mineral distribution and cellular alignment. For this purpose, wet-spun continuous microfibers are produced using polycaprolactone (PCL)/ gelatin and PCL/gelatin/hydroxyapatite nano-to-microparticles (HAp). Higher extrusion rates result in aligned PCL/gelatin microfibers while, in the case of PCL/gelatin/HAp, the presence of minerals leads to a less organized structure. Biological performance using human adipose-derived stem cells (hASCs) demonstrates that topography of PCL/gelatin microfibers can induce cytoskeleton elongation, resembling native tenogenic organization. Matrix mineralization on PCL/gelatin/HAp wet-spun composite microfibers suggests the production of an osteogenic-like matrix, without external addition of osteogenic medium supplementation. As proof of concept, a 3D gradient structure is produced by assembling PCL/gelatin and PCL/gelatin/HAp microfibers, resulting in a fibrous scaffold with a continuous topographical and compositional gradient. Overall, the feasibility of wet-spinning for the generation of continuously aligned and textured microfibers is demonstrated, which can be further assembled into more complex 3D gradient structures to mimic characteristic features of tendon-to-bone interfaces. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/60737 |
DOI: | 10.1002/adhm.201900200 |
ISSN: | 2192-2640 |
Versão da editora: | https://onlinelibrary.wiley.com/doi/full/10.1002/adhm.201900200 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19880-Calejo_et_al-2019-Advanced_Healthcare_Materials.pdf | 2,91 MB | Adobe PDF | Ver/Abrir |