Please use this identifier to cite or link to this item:

TitleBi-directional modulation of cellular interactions in an in vitro co-culture model of tendon-to-bone interface
Author(s)Calejo, I.
Costa-Almeida, R.
Gonçalves, A. I.
Berdecka, D.
Reis, R. L.
Gomes, Manuela E.
Keywordsadipose-derived stem cells
co-culture system
Enthesis regeneration
Interfacial tissue engineering
Osteogenic medium
Tendon-derived cells
Issue dateAug-2018
PublisherJohn Wiley & Sons
JournalCell Proliferation
CitationCalejo I., Costa-Almeida R., Gonçalves A. I., Berdecka D., Reis R. L., Gomes M. E. Bi-directional modulation of cellular interactions in an in vitro co-culture model of tendon-to-bone interface, Cell Proliferation, doi:10.1111/cpr.12493, 2018
Abstract(s)Objectives: This work aimed at studying in vitro interactions between human tendonâ derived cells (hTDCs) and preâ osteoblasts (preâ OBs) that may trigger a cascade of events involved in enthesis regeneration. Materials and methods The effect of 5 osteogenic medium (OM) conditions over the modulation of hTDCs and preâ OBs towards the tenogenic and osteogenic phenotypes, respectively, was studied. Three different medium conditions were chosen for subsequently establishing a direct coâ culture system in order to study the expression of bone, tendon and interfaceâ related markers. Results A higher matrix mineralization and ALP activity was observed in coâ cultures in the presence of OM. Higher transcription levels of boneâ (ALPL, RUNX2, SPP1) and interfaceâ related genes (ACAN, COMP) were found in coâ cultures. The expression of aggrecan was influenced by the presence of OM and cellâ cell interactions occurring in coâ culture. Conclusions The present work assessed both the influence of OM on cell phenotype modulation and the importance of coâ culture models while promoting cellâ cell interactions and the exchange of soluble factors in triggering an interfaceâ like phenotype to potentially modulate enthesis regeneration.
Publisher version
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
  Restricted access
2,27 MBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID