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https://hdl.handle.net/1822/58330
Título: | Biotechnological production of xylitol: engineering industrial Saccharomyces cerevisiae for valorization of lignocellulosic biomass |
Autor(es): | Sara Baptista Romaní, Aloia Cunha, Joana T. Domingues, Lucília |
Palavras-chave: | Biorefinery Xylitol Industrial Saccharomyces cerevisiae Fed-batch fermentation |
Data: | 24-Out-2018 |
Citação: | Sara Baptista; Romaní, Aloia; Cunha, Joana T.; Domingues, Lucília, Biotechnological production of xylitol: engineering industrial Saccharomyces cerevisiae for valorization of lignocellulosic biomass. 4-CIAB - Proceedings of the 4th Iberoamerican Congress on Biorefineries. Vol. Actas 48, Jaén, Spain, 24-26 October, 481-481, 2018. ISBN: 978-84-9159-144-3 |
Resumo(s): | The use of renewable biomass, such as lignocellulosic materials, for the production of biofuels and chemicals within a biorefinery scheme contributes to achieve a sustainable development. Xylitol has been identified as one of the top 12 value added chemicals to be obtained from biomass, and can be produced from hemicellulose-derived xylose through biotechnological processes [1]. In this work, xylitol was produced from xylose (using glucose as co-substrate for co-factor regeneration) in batch fermentations by the industrial Saccharomyces cerevisiae PE-2 strain (over)expressing (1) a wild type xylose reductase from Pichia spititis (XR); (2) a NADH-preferable xylose reductase mutant (mut-XR) from Pichia spititis and (3) the endogenous GRE3 gene which encodes for an unspecific aldose reductase (AR). Maximum yield (0.98 g g-1) was obtained by the strain overexpressing the GRE3 gene. Moreover, the recombinant strain PE-2-GRE3 showed significantly higher xylitol productivity than the laboratory strain, CENPK.113-5D overexpressing the same gene. This strain (PE-2-GRE3) was selected for bioconversion of 160 g L-1 of xylose in a fed-batch fermentation, which resulted in 149 g L-1 of xylitol concentration with a productivity of 1.2 g L-1 h-1. These results open new perspectives and opportunities for the valorisation of hemicellulosic hydrolysates through the production of xylitol within a biorefinery concept. |
Tipo: | Comunicação em painel |
URI: | https://hdl.handle.net/1822/58330 |
ISBN: | 978-84-9159-144-3 |
Versão da editora: | http://ceaema.ujaen.es/4CIAB/home-2/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_49428_1.pdf | 1,05 MB | Adobe PDF | Ver/Abrir | |
document_49428_2.pdf | 283,58 kB | Adobe PDF | Ver/Abrir |