Please use this identifier to cite or link to this item:
https://hdl.handle.net/1822/56319
Title: | Gellan Gum-based luminal fillers for peripheral nerve regeneration: an in vivo study in the rat sciatic nerve repair model |
Author(s): | Carvalho, C. R. Wrobel, S. Meyer, C. Branderberger, C. Cengiz, I. F. López-Cebral, R. Silva-Correia, J. Ronchi, J. Reis, R. L. Grothe, C. Oliveira, J. M. Haastert-Talini, K. |
Keywords: | Biomaterials Gellan Gum Peripheral Nerve Regeneration |
Issue date: | May-2018 |
Publisher: | Royal Society of Chemistry |
Journal: | Biomaterials Science |
Citation: | Carvalho C. R., Wrobel S., Meyer C., Branderberger C., Cengiz I. F., López-Cebral R., Silva-Correia J., Ronchi J., Reis R. L., Grothe C., Oliveira J. M., Haastert-Talini K. Gellan Gum-based luminal fillers for peripheral nerve regeneration: an in vivo study in the rat sciatic nerve repair model, BIOMATERIALS SCIENCE, Vol. 6, Issue 6, pp. 1059-1075, doi:10.1039/c7bm01101f, 2018 |
Abstract(s): | Peripheral nerve injuries (PNI) resulting in a gap to be bridged between the transected nerve ends are commonly reconstructed with autologous nerve tissue, but there is a need for valuable alternatives. This experimental work considers the innovative use of the biomaterial Gellan Gum (GG) as a luminal filler for nerve guidance channels made from chitosan with a 5% degree of acetylation. The engineered constructs should remodel the structural support given to regenerating axons by the so-called bands of Büngner. Four different GG formulations were produced by combining varying amounts of High-Acyl GG (HA-GG) and Methacrylated GG (MA-GG). The effective porosity of the freeze-dried networks was analysed by SEM and micro-CT 3D reconstructions, while the degradation and swelling abilities were characterized in vitro for up to 30 days. The metabolic activity and viability of immortalized Schwann cells seeded onto the freeze-dried networks were also evaluated. Finally, the developed hydrogel formulations were freezedried within the chitosan nerve guides and implanted in a 10 mm rat sciatic nerve defect. Functional and histomorphological analyses after 3, 6, and 12 weeks in vivo revealed that although it did not result in improved nerve regeneration, the NGC25:75 formulations could provide a basis for further development of GG scaffolds as luminal fillers for hollow nerve guidance channels. |
Type: | Article |
URI: | https://hdl.handle.net/1822/56319 |
DOI: | 10.1039/c7bm01101f |
ISSN: | 45343 |
Publisher version: | http://pubs.rsc.org/en/content/articlehtml/2018/BM/C7BM01101F |
Peer-Reviewed: | yes |
Access: | Open access |
Appears in Collections: | 3B’s - Artigos em revistas/Papers in scientific journals |
Files in This Item:
File | Description | Size | Format | |
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19530-final PDF C7BM01101F.pdf | 745,73 kB | Adobe PDF | View/Open |