Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/56285

TitleKefiran biopolymer: Evaluation of its physicochemical and biological properties
Author(s)Radhouani, H.
Gonçalves, C.
Maia, F. R.
Oliveira, J. M.
Reis, R. L.
Keywordscytotoxicity
Drug Delivery System
enzymatic resistance
Exopolysaccharide Kefiran
physicochemical characterization
Portuguese kefir grains
Issue dateAug-2018
PublisherSAGE
JournalJournal of Bioactive and Compatible Polymers
CitationRadhouani H., Gonçalves C., Maia F. R., Oliveira J. M., Reis R. L. Kefiran biopolymer: Evaluation of its physicochemical and biological properties, Journal Of Bioactive And Compatible Polymers, Vol. 33, Issue 5, pp. 461-478, doi:10.1177/0883911518793914, 2018
Abstract(s)Kefiran, an exopolysaccharide produced by lactic acid bacteria, has received a great interest due to a variety of health claims. In this study, we aim to investigate the physicochemical and biological properties of Kefiran polysaccharide extracted from Portuguese kefir grains. The kefir growth rate was about 56% (w/w) at room temperature and the kefir pH after 24 h was about 4.6. The obtained yield of Kefiran polysaccharide extracted from the kefir grains was about 4.26% (w/w). The Kefiran structural features were showed in the 1H nuclear magnetic resonance spectrum. The bands observed in the infrared spectrum confirmed that the Kefiran had a β-configuration; and the X-ray photoelectron spectroscopy analysis confirmed the structure and composition of Kefiran and revealed a C/O atomic ratio of 1.46. Moreover, Kefiran showed an average molecular weight (Mw) of 534 kDa and a number-average molecular weight (Mn) of 357 kDa. Regarding the rheological data obtained, Kefiran showed an interesting adhesive performance accompanied by a pseudoplastic behavior, and the extrusion force of Kefiran was 1 N. Furthermore, Kefiran exhibited a higher resistance to hyaluronidase degradation than hyaluronic acid. Finally, Kefiran showed a lack of cytotoxic response through its ability to support metabolic activity and proliferation of L929 cells, and had no effect on these cells’ morphology. Our research suggested that Kefiran polymer has attractive and interesting properties for a wide range of biomedical applications, such as tissue engineering and regenerative medicine.
TypeArticle
URIhttp://hdl.handle.net/1822/56285
DOI10.1177/0883911518793914
ISSN0883-9115
Publisher versionhttp://journals.sagepub.com/doi/abs/10.1177/0883911518793914?journalCode=jbca
Peer-Reviewedyes
AccessOpen access
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

Files in This Item:
File Description SizeFormat 
19595-Publication 1_Radhouani et al., 2018.pdf664,85 kBAdobe PDFView/Open

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID