Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/53720

Title3D mimicry of native-tissue-fiber architecture guides tendon-derived cells and adipose stem cells into artificial tendon constructs
Author(s)Laranjeira, Mariana
Domingues, Rui Miguel Andrade
Almeida, Raquel Carvalho Ferreira Costa
Reis, R. L.
Gomes, Manuela E.
Keywords3D hierarchical scaffolds
Nanotopography
Tendons
Tenogenic differentiation
Tissue engineering
Issue date18-Aug-2017
PublisherWiley
JournalSmall
Abstract(s)Tendon and ligament (T/L) function is intrinsically related with their unique hierarchically and anisotropically organized extracellular matrix. Their natural healing capacity is, however, limited. Here, continuous and aligned electrospun nanofiber threads (CANT) based on synthetic/natural polymer blends mechanically reinforced with cellulose nanocrystals are produced to replicate the nanoscale collagen fibrils grouped into microscale collagen fibers that compose the native T/L. CANT are then incrementally assembled into 3D hierarchical scaffolds, resulting in woven constructions, which simultaneously mimic T/L nano-to-macro architecture, nanotopography, and nonlinear biomechanical behavior. Biological performance is assessed using human-tendon-derived cells (hTDCs) and human adipose stem cells (hASCs). Scaffolds nanotopography and microstructure induce a high cytoskeleton elongation and anisotropic organization typical of tendon tissues. Moreover, the expression of tendon-related markers (Collagen types I and III, Tenascin-C, and Scleraxis) by both cell types, and the similarities observed on their expression patterns over time suggest that the developed scaffolds not only prevent the phenotypic drift of hTDCs, but also trigger tenogenic differentiation of hASCs. Overall, these results demonstrate a feasible approach for the scalable production of 3D hierarchical scaffolds that exhibit key structural and biomechanical properties, which can be advantageously explored in acellular and cellular T/L TE strategies.
TypeArticle
URIhttps://hdl.handle.net/1822/53720
DOI10.1002/smll.201700689
ISSN1613-6810
e-ISSN1613-6829
Publisher versionhttps://onlinelibrary.wiley.com/doi/abs/10.1002/smll.201700689
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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