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TitleA 1H-NMR-based metabolomic analysis of propolis from Santa Catarina state
Author(s)Somensi-Zeggio, A. R.
Tomazzoli, M. M.
Kobe, S. O.
Zeri, A. C. M.
Sforça, M.
Rocha, Miguel
Carreira, Rafael
Maraschin, M.
Issue date5-Aug-2012
CitationSomensi-Zeggio, A. R.; Tomazzoli, M. M.; Kobe, S. O.; Zeri, A. C. M.; Sforça, M.; Rocha, Miguel; Carreira, Rafael; Maraschin, M., A 1H-NMR-based metabolomic analysis of propolis from Santa Catarinastate. 16th IUFoST World Congress of Food Science and Technology: Addressing Global Food Security and Wellness through Food Science and Technology. Foz do Iguaçu, Parana, Brazil, Aug 5-9, 2012.
Abstract(s)Propolis is a resinous biomass produced by honeybees from exudates of local flora. It has been used since ancient times in folk medicine and in recent years has been added to foods and beverages to improve health and prevent diseases. The chemical composition of propolis is highly variable and depends on the climate, season, specie of bee, and mainly the local flora visited by bees to collect resin. In order to identify groups of chemical similarity among samples (n=20 autumn, n=16 winter, n=19 spring, n=17 summer) of propolis produced in Santa Catarina (SC) state (southern Brazil - 2010), lyophilized ethanolic extracts (200 mg/ml, EtOH 70%, v/v) were solubilized in MeOD3 (700l) and analyzed by NMR spectroscopy. One-dimensional 1HNMR spectra were acquired at a magnetic field strength of 500,13/125,03 MHz using a Varian Inova 500 MHz equipment and standard conditions of data acquisition. The 1H-NMR peak list data set was processed under MetaboAnalyst 2.0. suite, computing the resonances at 0.80- 12ppm spectral window. Principal Components Analysis (PCA) score scatter plots (PC1 88.2% x PC2 2.2%) clearly demonstrated samples discriminated mainly according to the season of production. These results suggest that not only geographical origin is important for the classification of propolis, but the seasonal effects as well. Since seasons directly influence the flora available from where bees collect resin, the propolis chemical profile can be significantly modified over the seasons even from a same geographical origin.
Description16th IUFoST World Congress of Food Science and Technology: Addressing Global Food Security and Wellness through Food Science and Technology
Publisher version
AccessOpen access
Appears in Collections:CEB - Resumos em Livros de Atas / Abstracts in Proceedings

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