Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/43825

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dc.contributor.authorMartins, Margarida Isabel Barros Coelhopor
dc.contributor.authorRibeiro, Diana Margarida da Costapor
dc.contributor.authorMartins, Albinopor
dc.contributor.authorReis, R. L.por
dc.contributor.authorNeves, N. M.por
dc.date.accessioned2016-12-23T10:23:36Z-
dc.date.available2016-12-23T10:23:36Z-
dc.date.issued2016-03-
dc.date.submitted2016-03-
dc.identifier.citationMartins M., Ribeiro D., Martins A., Reis R. L., Neves N. M. Extracellular Vesicles Derived from Osteogenically Induced Human Bone Marrow Mesenchymal Stem Cells Can Modulate Lineage Commitment, Stem Cell Reports , Vol. 6, Issue 3, pp. 284-291, doi:10.1016/j.stemcr.2016.01.001, 2016por
dc.identifier.issn2213-6711por
dc.identifier.urihttps://hdl.handle.net/1822/43825-
dc.description.abstractThe effective osteogenic commitment of human bone marrow mesenchymal stem cells (hBMSCs) is critical for bone regenerative therapies. Extracellular vesicles (EVs) derived from hBMSCs have a regenerative potential that has been increasingly recognized. Herein, the osteoinductive potential of osteogenically induced hBMSC-EVs was examined. hBMSCs secreted negatively charged nanosized vesicles (⠼35 nm) with EV-related surface markers. The yield of EVs over 7 days was dependent on an osteogenic stimulus (standard chemical cocktail or RUNX2 cationic-lipid transfection). These EVs were used to sequentially stimulate homotypic uncommitted cells during 7 days, matching the seeding density of EV parent cells, culture time, and stimuli. Osteogenically committed hBMSC-EVs induced an osteogenic phenotype characterized by marked early induction of BMP2, SP7, SPP1, BGLAP/IBSP, and alkaline phosphatase. Both EV groups outperformed the currently used osteoinductive strategies. These data show that naturally secreted EVs can guide the osteogenic commitment of hBMSCs in the absence of other chemical or genetic osteoinductors.por
dc.description.sponsorshipThe authors thank the financial support of QREN (RL1-ABMRNORTE-01-0124-FEDER-000016 and RL3–TECT–NORTE-01-0124- FEDER-000020) co-financed by North Portugal Regional Operational Program (ON.2–O Novo Norte), under the NSRF, through the ERDF; the European Union’s Seventh Framework Program (FP7/2007–2013), Grant No. REGPOT-CT2012-316331- POLARIS; and the laboratory assistance of Elsa Ribeiro, Magda Graça, and Sónia Zacarias.por
dc.language.isoengpor
dc.publisherCell Presspor
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/316331/EU-
dc.rightsopenAccesspor
dc.subjectAdvanced therapies/ bone regenerationpor
dc.subjectDifferentiationpor
dc.subjectExosomepor
dc.subjectOsteogenic commitment strategiespor
dc.subjectSecretomepor
dc.subjectStem cellspor
dc.titleExtracellular vesicles derived from osteogenically induced human bone marrow mesenchymal stem cells can modulate lineage commitmentpor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://www.cell.com/stem-cell-reports/pdf/S2213-6711(16)00002-3.pdfpor
dc.commentshttp://3bs.uminho.pt/node/18674por
sdum.publicationstatusinfo:eu-repo/semantics/publishedVersionpor
oaire.citationStartPage284por
oaire.citationEndPage291por
oaire.citationIssue3por
oaire.citationTitleStem Cell Reportspor
oaire.citationVolume6por
dc.date.updated2016-12-14T15:34:50Z-
dc.identifier.doi10.1016/j.stemcr.2016.01.001por
dc.identifier.pmid26923821por
dc.subject.wosScience & Technologypor
sdum.journalStem Cell Reportspor
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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