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https://hdl.handle.net/1822/42995
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Campo DC | Valor | Idioma |
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dc.contributor.author | Almeida, Raquel Costa | por |
dc.contributor.author | Carvalho, Daniel T. O. | por |
dc.contributor.author | Ferreira, Miguel J. S. | por |
dc.contributor.author | Aresta, Guilherme | por |
dc.contributor.author | Gomes, Manuela E. | por |
dc.contributor.author | Loon, Jack J. W. A. van | por |
dc.contributor.author | Heiden, Kim van der | por |
dc.contributor.author | Granja, Pedro L. | por |
dc.date.accessioned | 2016-11-11T12:11:57Z | - |
dc.date.issued | 2016 | - |
dc.date.submitted | 2016-01 | - |
dc.identifier.citation | Costa-Almeida R., Carvalho D. T. O., Ferreira M. J. S., Aresta G., Gomes M. E., van Loon J. J. W. A., Van der Heiden K., Granja P. L. Effects of hypergravity on the angiogenic potential of endothelial cells, Journal Of The Royal Society Interface, Vol. 13, doi:10.1098/rsif.2016.0688, 2016 | por |
dc.identifier.issn | 1742-5662 | por |
dc.identifier.uri | https://hdl.handle.net/1822/42995 | - |
dc.description.abstract | Angiogenesis, the formation of blood vessels from pre-existing ones, is a key event in pathology, including cancer progression, but also in homeostasis and regeneration. As the phenotype of endothelial cells (ECs) is continuously regulated by local biomechanical forces, studying endothelial behaviour in altered gravity might contribute to new insights towards angiogenesis modu- lation. This study aimed at characterizing EC behaviour after hypergravity exposure (more than 1g), with special focus on cytoskeleton architecture and capillary-like structure formation. Herein, human umbilical vein ECs (HUVECs) were cultured under two-dimensional and three-dimensional conditions at 3g and 10g for 4 and 16 h inside the large diameter centrifuge at the European Space Research and Technology Centre (ESTEC) of the European Space Agency. Although no significant tendency regarding cyto- skeleton organization was observed for cells exposed to high gâ s, a slight loss of the perinuclear localization of b-tubulin was observed for cells exposed to 3g with less pronounced peripheral bodies of actin when compared with 1g control cells. Additionally, hypergravity exposure decreased the assembly of HUVECs into capillary-like structures, with a 10g level significantly reducing their organization capacity. In conclusion, short-term hypergravity seems to affect EC phenotype and their angiogenic potential in a time and g-level-dependent manner. | por |
dc.description.sponsorship | This work was supported by the Spin Your Thesis! 2014 programme, organized by ESA Education Office. Additional funding include the European Regional Development Fund (ERDF) through the Programa Operacional Factores de Competitividade – COMPETE, and by Portuguese funds through FCT-Fundação para a Ciência e a Tecnologia in the framework of FCT-POPH-FSE, the research grant PEst-C/SAU/LA0002/2011, and co-financed by the North Portugal Regional Operational Programme (ON.2-O Novo Norte) in the framework of the project NORTE-01-0145-FEDER-000012, under the National Strategic Reference Framework (NSRF). Acknowledgements. The experiments reported here were performed in the framework of the Spin Your Thesis! 2014 programme, organized by ESA Education Office. The authors are grateful to staff of ESA Education Office, Natacha Callens and Lily Ha, for support, and to Alan Dowson for the technical support before and during the campaign. R.C.-A. acknowledges the PhD grant SFRH/BD/96593/2013 from FCT-Fundação para a Ciência e a Tecnologia | por |
dc.language.iso | eng | por |
dc.publisher | Royal Society | por |
dc.relation | info:eu-repo/grantAgreement/FCT/6820 - DCRRNI ID/PEst-C%2FSAU%2FLA0002%2F2011/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F96593%2F2013/PT | - |
dc.rights | restrictedAccess | por |
dc.subject | Altered gravity | por |
dc.subject | Capillary-like structures | por |
dc.subject | Cytoskeleton | por |
dc.subject | Hypergravity pre-stimulation | por |
dc.subject | Three-dimensional matrigel | por |
dc.title | Effects of hypergravity on the angiogenic potential of endothelial cells | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://rsif.royalsocietypublishing.org/content/13/124/20160688 | por |
dc.comments | http://3bs.uminho.pt/node/18892 | por |
sdum.publicationstatus | info:eu-repo/semantics/publishedVersion | por |
oaire.citationIssue | 124 | por |
oaire.citationTitle | Journal of the Royal Society Interface | por |
oaire.citationVolume | 13 | por |
dc.date.updated | 2016-11-10T08:59:51Z | - |
dc.identifier.doi | 10.1098/rsif.2016.0688 | por |
dc.identifier.pmid | 28334696 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of the Royal Society Interface | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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18892-Effects of hypergravity on the angiogenic potential of endothelial cells.pdf Acesso restrito! | 1,49 MB | Adobe PDF | Ver/Abrir |