Polyphasic identification of Aspergillus section Nigri preserved under mineral oil at URM culture collection

Abstract

[Excerpt] The species of Aspergillus section Nigri are isolated from different environments. However, úe main habitat of úese species is the soil. According Samson et al. (2007) there are 19 species of Aspergil/zs section Nigri accepted. The species must be delineated based on a polyphasic approach, including morphology, physiology, profile of secondary metabolites and molecular biology (Samson and Varga,2009). Additionally, according to Santos et al. (2010a,2010b) it is clearer that spectral analyses add value to the polyphasic approach. It generates quahty data which are accurate and useful when some of the methods described above presented limitations. Matrix-Assisted Laser Desorptiorúonisation Time-Of-Flight Intact Cell Mass Spectrometry (MALDI-TOF ICMS) is a spectral technique that analyses the chemical cellular composition of microorganisms providing rapid and discriminatory fingerprints for identification.