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https://hdl.handle.net/1822/31998
Título: | Modulation of crude glycerol fermentation byClostridium pasteurianum DSM 525 towards theproduction of butanol |
Autor(es): | Gallardo, R. Alves, M. M. Rodrigues, L. R. |
Palavras-chave: | Butanol Clostridium pasteurianum Crude glycerol Butyrate 1,3-Propanediol |
Data: | 2014 |
Editora: | Pergamon Press Ltd. |
Revista: | Biomass and Bioenergy |
Resumo(s): | High production yields and productivities are requisites for the development of an industrial butanol production process based on biodiesel-derived crude glycerol. However, impurities present in this substrate and/or the concentration of glycerol itself can affect the microbial metabolism. In this work, the effect of crude glycerol concentration on the production of butanol and 1,3-propanediol (1,3-PDO) by Clostridium pasteurianum DSM 525 is studied. Also, the effect of acetate and butyrate supplementation to the culture medium and the culture medium composition are evaluated. The results showed a marked effect of crude glycerol concentration on the product yield. The competitive nature of butanol and 1,3-PDO pathways has been evident, and a shift to the butanol pathway once using higher substrate concentrations (up to 35 g l 1) was clearly observed. Butyrate supplementation to the culture medium resulted in a 45% higher butanol titre, a lower production of 1,3-PDO and it decreased the fermentation time. Acetate supplementation also increased the butanol titre but the fermentation was longer. Even though glycerol consumption could not be increased over 32 g l 1, when the concentrations of NH4Cl and FeCl2 were simultaneously increased, the results obtained were similar to those observed when butyrate was supplemented to the culture medium; a 35% higher butanol yield at the expense of 1,3-PDO and a shorter fermentation. The results herein gathered suggest that there are other factors besides butanol inhibition and nutrient limitation that affect the glycerol consumption. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/31998 |
DOI: | 10.1016/j.biombioe.2014.10.015 |
ISSN: | 0961-9534 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_18494_1.pdf | 676,8 kB | Adobe PDF | Ver/Abrir |