Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/31998
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Gallardo, R. | por |
dc.contributor.author | Alves, M. M. | por |
dc.contributor.author | Rodrigues, L. R. | por |
dc.date.accessioned | 2014-12-15T18:57:28Z | - |
dc.date.available | 2014-12-15T18:57:28Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0961-9534 | por |
dc.identifier.uri | https://hdl.handle.net/1822/31998 | - |
dc.description.abstract | High production yields and productivities are requisites for the development of an industrial butanol production process based on biodiesel-derived crude glycerol. However, impurities present in this substrate and/or the concentration of glycerol itself can affect the microbial metabolism. In this work, the effect of crude glycerol concentration on the production of butanol and 1,3-propanediol (1,3-PDO) by Clostridium pasteurianum DSM 525 is studied. Also, the effect of acetate and butyrate supplementation to the culture medium and the culture medium composition are evaluated. The results showed a marked effect of crude glycerol concentration on the product yield. The competitive nature of butanol and 1,3-PDO pathways has been evident, and a shift to the butanol pathway once using higher substrate concentrations (up to 35 g l 1) was clearly observed. Butyrate supplementation to the culture medium resulted in a 45% higher butanol titre, a lower production of 1,3-PDO and it decreased the fermentation time. Acetate supplementation also increased the butanol titre but the fermentation was longer. Even though glycerol consumption could not be increased over 32 g l 1, when the concentrations of NH4Cl and FeCl2 were simultaneously increased, the results obtained were similar to those observed when butyrate was supplemented to the culture medium; a 35% higher butanol yield at the expense of 1,3-PDO and a shorter fermentation. The results herein gathered suggest that there are other factors besides butanol inhibition and nutrient limitation that affect the glycerol consumption. | - |
dc.description.sponsorship | The authors acknowledge the financial support from the Strategic Project PEst-OE/EQB/LA0023/2013; the project ref. RECI/BBB-EBI/0179/2012 (project number FCOMP-01-0124-FEDER-027462); and the PhD grant given to R. Gallardo (ref SFRH/BD/42900/2008) funded by Fundacao para a Ciencia e a Tecnologia. The authors thank the MIT-Portugal Program for the support given to R. Gallardo. | por |
dc.language.iso | eng | por |
dc.publisher | Pergamon Press Ltd. | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876-PPCDTI/126270/PT | - |
dc.rights | openAccess | por |
dc.subject | Butanol | por |
dc.subject | Clostridium pasteurianum | por |
dc.subject | Crude glycerol | por |
dc.subject | Butyrate | por |
dc.subject | 1,3-Propanediol | por |
dc.title | Modulation of crude glycerol fermentation byClostridium pasteurianum DSM 525 towards theproduction of butanol | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.comments | CEB18494 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 134 | por |
oaire.citationEndPage | 143 | por |
oaire.citationConferencePlace | United Kingdom | - |
oaire.citationTitle | Biomass and Bioenergy | por |
oaire.citationVolume | 71 | por |
dc.date.updated | 2014-12-01T10:32:11Z | - |
dc.identifier.doi | 10.1016/j.biombioe.2014.10.015 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biomass and Bioenergy | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_18494_1.pdf | 676,8 kB | Adobe PDF | Ver/Abrir |