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https://hdl.handle.net/1822/31607
Título: | Solid-state cultivation of fungal strains P79 on spent coffee grounds for the extraction of antioxidant phenolic compounds and proteins |
Autor(es): | Machado, Ercília M. S. Teixeira, J. A. Mussatto, Solange I. |
Data: | 2012 |
Editora: | Universidade do Minho. Centro de Engenharia Biológica (CEB) |
Citação: | Machado, E. M. S.; Teixeira, José A.; Mussatto, Solange I., Solid-state cultivation of fungal strains P79 on spent coffee grounds for the extraction of antioxidant phenolic compounds and proteins. ECCO XXXI Meeting, Abstracts Book - Biological Resource Centres: Closing the gap between science and society. No. P79, Braga, Portugal, 14-15 Junho, Micoteca da Universidade do Minho, 141-1412012. . ISBN: 978-972-97916-5-9 |
Resumo(s): | Four fungal strains (Aspergillus ustus PSS, Mucor sp. 3P, Penicillium purpurogenum GH2 and Neurospora crassa ATCC10337) were cultivated under solid-state conditions on spent coffee grounds (SCG) aiming to extract antioxidant phenolic compounds and proteins. Cultivations were performed at 30 ºC for 6 days. The produced extracts were characterized regarding the contents of total phenolic compounds, flavonoids, proteins, and antioxidant activity, which was determined by two methods (ferric reducing antioxidant power (FRAP) assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH) method). N. crassa released the highest contents of phenolic compounds (10.99 mg gallic acid equivalents/g SCG) and 0.83 mg protein/g SCG. P. purpurogenum released the highest contents of protein (0.90 mg/g SCG) and flavonoids (0.53 mg quercetin/g SCG) from this raw material. In terms of antioxidant activity, the extract produced by Mucor sp. presented the highest values either by the FRAP (0.047 mM Fe(II)/g SCG) and DPPH (81.9%) methods, and the extract produced by A. ustus also contained elevated antioxidant activity by the DPPH method (81.6%). These results reveal that the studied fungal strains have different ability to degrade SCG, and the selection among them must be done based on the product that is desired to obtain. Solid-state cultivation with fungi was proved to be a useful and environmentally-friendly alternative to extract valuable compounds from SCG. |
Tipo: | Resumo em ata de conferência |
URI: | https://hdl.handle.net/1822/31607 |
ISBN: | 978-972-97916-5-9 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
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document_18179_1.pdf | 91,29 kB | Adobe PDF | Ver/Abrir |