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https://hdl.handle.net/1822/31516
Título: | Silk fibroin nanoparticles for drug delivery purposes : stabilization, incorporation and release design |
Autor(es): | Ferreira, A. V. Volkov, Vadim Vasconcelos, Andreia Abreu, Aana S. Botelho, C. M. Paulo, Artur Cavaco |
Data: | 25-Mai-2014 |
Citação: | Ferreira, Ana; Vasconcelos, Andreia; Abreu, A. S.; Botelho, C. M.; Paulo, Artur Cavaco, Silk Fibroin Nanoparticles for Drug Delivery purposes: Stabilization, Incorporation and Release Design. IPFB2014 - 8th International Conference on Polymer and Fiber Biotechnology. Braga, Portugal, May 25-27, 2014. |
Resumo(s): | Silk Fibroin (SF) has been extensively studied for various applications due to its impressive mechanical properties and biocompatibility. Recently, SF based-particles have been proposed as controlled drug delivery systems. A new and efficient method was developed to prepare SF nanoparticles (SF-NPs) by high pressure homogenization (HPH) emulsification, in oil-in-water emulsions (o/w). During the NPs production by HPH emulsification process, the secondary SF structure changed from random-coil conformation to a more stable structure, -sheets. To improve even more the NPs stability over time the effect of various surfactants was studied, namely poloxamer 407, transcutol, tween 80 and sodium dodecyl sulfate, in which SF nanoemulsions with 1% of transcutol demonstrated lower diameters and better polydispersity values during the 4 weeks of evaluation. The drug incorporation efficiency and release of SF-NPs was assessed using orange IV dye as model-drug. The influence of a human protease (human neutrophil elastase) on orange IV release profile was also evaluated. The encapsulation of orange IV effectively stabilized the size and size distribution of the SF-NPs over time, being evident the conformational change to -sheets. SF-NPs encapsulated with orange IV had a formation and encapsulation efficiency of 67% and 91%, respectively, with a controlled release over time. The stability and release profile induced by the SF-NPs enhances its potential for various applications, including biomedical. |
Tipo: | Resumo em ata de conferência |
URI: | https://hdl.handle.net/1822/31516 |
Versão da editora: | http://ipfb2014.wix.com/ipfb2014 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
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Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_18604_1.pdf | 94,08 kB | Adobe PDF | Ver/Abrir |