Evaluation of macroporous and microporous carriers for cho-k1 cell growth and mab production

Abstract

Large-scale biopharmaceutical production commonly relies on suspension cell cultures that provide higher yields than adherent cultures. However, most mammalian cells grow adherently and need to be adapted to suspension, which is not always simple or feasible. Microcarrier culture introduces new possibilities and allows the practical high yield culture of anchorage-dependent cells in suspension systems. In these systems, cells grow as monolayers on the surface of the microcarriers, which are usually in suspension in the culture medium by gentle rocking. The aim of the study was to evaluate, compare and optimize the use of microcarriers for the growth and antibody production by CHO-K1 cells.