Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/30303
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Amorim, Sara | por |
dc.contributor.author | Martins, Albino | por |
dc.contributor.author | Neves, N. M. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Pires, R. A. | por |
dc.date.accessioned | 2014-09-29T14:43:49Z | - |
dc.date.available | 2014-09-29T14:43:49Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 2050-750X | por |
dc.identifier.uri | https://hdl.handle.net/1822/30303 | - |
dc.description | First published online 15 Aug 2014 | por |
dc.description.abstract | Herein, we evaluate the influence of a PLL–HA bilayer on the surface of silica nanoparticles on their capacity to induce the osteogenic differentiation of human bone marrow stem cells (hBMSCs), as a function of their concentration (50 μg mL−1, 25 μg mL−1 and 12.5 μg mL−1). To this purpose, we synthesized silica nanoparticles (diameter of 250 nm; ζ-potential of −25 mV) that were coated with PLL–HA (diameter of 560 nm; ζ-potential of −35 mV). The cell viability, cell proliferation, protein quantification (i.e. MTS, DNA and ALP, respectively) and gene expression (of osteogenesis-related genes: ALP, osteocalcin, collagen type I, bone sialoprotein, Runx-2, osteopontin and osterix) were monitored, for 21 days. We observed the overexpression of most of the tested osteogenic transcripts in the hBMSCs cultured with SiO2–PLL–HA, at concentrations of 25 μg mL−1 and 12.5 μg mL−1. These results indicate that the proposed nanoparticles temporarily improve the osteogenic differentiation of hBMSCs at low nanoparticle concentrations. | por |
dc.description.sponsorship | We acknowledge funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement number REGPOT-CT2012-316331-POLARIS. AM acknowledges QREN (project "RL1-ABMR-NORTE-01-0124-FEDER-000016" co-financed by the North Portugal Regional Operational Programme (ON. 2, "O Novo Norte") under the NSRF through the ERDF) for financing this research work. | por |
dc.language.iso | eng | por |
dc.publisher | Royal Society of Chemistry | por |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/316331/EU | - |
dc.rights | openAccess | por |
dc.subject | Hyaluronic acid | por |
dc.subject | Layer-by-layer | por |
dc.subject | Osteogenesis | por |
dc.title | Hyaluronic acid/poly-L-lysine bilayered silica nanoparticles enhance the osteogenic differentiation of human mesenchymal stem cells | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://pubs.rsc.org/en/content/articlepdf/2014/tb/c4tb01071j | por |
dc.comments | http://www.3bs.uminho.pt/node/18098 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 6939 | por |
oaire.citationEndPage | 6946 | por |
oaire.citationIssue | 40 | por |
oaire.citationTitle | Journal of materials chemistry B | por |
oaire.citationVolume | 2 | por |
dc.date.updated | 2014-09-12T09:41:40Z | - |
dc.identifier.doi | 10.1039/c4tb01071j | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of materials chemistry B | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
18098-c4tb01071j.pdf | 1,04 MB | Adobe PDF | Ver/Abrir |