Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/25180
Título: | Interactions between exogenous FGF-2 and sulfonic groups: in situ characterization and impact on the morphology of human adipose-derived stem cells |
Autor(es): | Amorim, Sara Pires, R. A. Costa, Diana Soares da Reis, R. L. Pashkuleva, I. |
Palavras-chave: | Adipose-derived stem cells FGF-2 Quartz crystal microbalance Self-assembled monolayers Streaming potential |
Data: | Mai-2013 |
Editora: | American Chemical Society |
Revista: | Langmuir |
Resumo(s): | FGF-2 is often used as a supplement to stem cells culture medium aiming at preserving their self-renewal capacity and plasticity through the passages. However, little is known on the influence of the underlying substrate in these interactions. In this study, we have used mixed self-assembled monolayers with different ratios of −SO3H and −OH tail groups to investigate the influence of substrate properties (e.g., charge) on the FGF-2 adsorption and activity. QCM-D data demonstrated that, in the presence of −OH groups, the quantity of the adsorbed FGF-2 is proportional to the percentage of surface −SO3H groups. The bioactivity of the adsorbed FGF-2 follows the same tendency as demonstrated by its interactions with anti-FGF-2. Surprisingly, the adlayer of FGF-2 formed on the surface containing only SO3H-tailed SAMs was similar to the surface with 25% of −SO3H groups, demonstrating that FGF-2 adsorption is not solely driven by electrostatic interactions. We related these results with changes in the morphology of adipose-derived stem cells (ASCs) cultured on the same surfaces. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/25180 |
DOI: | 10.1021/la400871c |
ISSN: | 0743-7463 |
Versão da editora: | http://pubs.acs.org/doi/abs/10.1021/la400871c |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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17554-Amorim et al_Langmuir_2013.pdf Acesso restrito! | 1,47 MB | Adobe PDF | Ver/Abrir |