Please use this identifier to cite or link to this item: http://hdl.handle.net/1822/24962

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dc.contributor.authorPereira, R. C.-
dc.contributor.authorScaranari, M.-
dc.contributor.authorBenelli, R.-
dc.contributor.authorStrada, P.-
dc.contributor.authorReis, R. L.-
dc.contributor.authorCancedda, Ranieri-
dc.contributor.authorGentili, Chiara-
dc.date.accessioned2013-08-09T14:57:56Z-
dc.date.available2013-08-09T14:57:56Z-
dc.date.issued2013-04-
dc.date.submitted2013-06-
dc.identifier.issn2152-4947por
dc.identifier.urihttp://hdl.handle.net/1822/24962-
dc.description.abstractPlatelet-rich plasma (PRP), a cocktail of platelet growth factors and bioactive proteins, has been proposed as a therapeutic agent to restore damaged articular cartilage. We report the biological effect of the platelet lysate (PL), a PRP derivative, on primary human articular chondrocytes cultured under both physiological and inflammatory conditions. When added to the culture medium, PL induced a strong mitogenic response in the chondrocytes. The in vitro expanded cell population maintained a chondrogenic redifferentiation potential as revealed by micromass culture in vitro and ectopic cartilage formation in vivo. Further, in chondrocytes cultured in the presence of the proinflammatory cytokine interleukin-1a (IL-1a), the PL induced a drastic enhancement of the synthesis of the cytokines IL-6 and IL-8 and of neutrophil-gelatinase associated lipocalin, a lipocalin expressed during chondrocyte differentiation and inflammation. These events were mediated by the p38 MAP kinase and NF-kB pathways. We observed that inflammatory stimuli activated phospo-MAP kinase-activated protein kinase 2, a direct target of p38. The proinflammatory effect of the PL was a transient phenomenon; after an initial upregulation, we observed significant reduction of the NF-kB activity together with the repression of the inflammatory enzyme cyclooxygenase-2. Moreover, the medium of chondrocytes cultured in the simultaneous presence of PL and IL-1a, showed a significant enhancement of the chemoattractant activity versus untreated chondrocytes. Our findings support the concept that the platelet products have a direct beneficial effect on articular chondrocytes and could drive in sequence a transient activation and the resolution of the inflammatory process, thus providing a rational for their use as therapeutic agents in cartilage inflammation and damage.por
dc.description.sponsorshipThe work was supported by institutional funds from the University of Genova and by European Union Projects (Angioscaff and GAMBA) and by a grant from Compagnia di San Paolo, Torino. The authors would like to thank Dr. Fiorella Descalzi and Dr. Maddalena Mastrogiacomo of our institute for helpful discussions and suggestions, Dr. Michele Grandizio for providing the biological samples, and the medical staff of the Recco Hospital (Recco, Genova, Italy) for their collaboration and support.por
dc.language.isoengpor
dc.publisherMary Ann Liebertpor
dc.rightsrestrictedAccesspor
dc.subjectHuman articular chondrocytespor
dc.subjectInflammationpor
dc.titleDual effect of platelet lysate on human articular cartilage : a maintenance of chondrogenic potential and a transient pro-inflammatory activity followed by an inflammation resolutionpor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttp://online.liebertpub.com/doi/abs/10.1089/ten.TEA.2012.0225por
dc.commentshttp://www.3bs.uminho.pt/node/17628por
sdum.publicationstatuspublishedpor
oaire.citationStartPage1476por
oaire.citationEndPage1488por
oaire.citationIssue11-12por
oaire.citationTitleTissue Engineering : part Apor
oaire.citationVolume19por
dc.date.updated2013-08-05T09:58:18Z-
dc.identifier.doi10.1089/ten.tea.2012.0225por
dc.identifier.pmid23360471por
dc.subject.wosScience & Technologypor
sdum.journalTissue Engineering : part Apor
Appears in Collections:3B’s - Artigos em revistas/Papers in scientific journals

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