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Campo DCValorIdioma
dc.contributor.authorMendes, L. F.-
dc.contributor.authorPirraco, Rogério P.-
dc.contributor.authorSzymczyk, Wojciech-
dc.contributor.authorFrias, A. M.-
dc.contributor.authorSantos, T. C.-
dc.contributor.authorReis, R. L.-
dc.contributor.authorMarques, A. P.-
dc.date.accessioned2013-04-10T13:37:21Z-
dc.date.available2013-04-10T13:37:21Z-
dc.date.issued2012-07-
dc.date.submitted2012-07-
dc.identifier.issn1932-6203por
dc.identifier.urihttps://hdl.handle.net/1822/23643-
dc.description.abstractIn recent years several studies have been supporting the existence of a close relationship in terms of function and progeny between Mesenchymal Stem Cells (MSCs) and Pericytes. This concept has opened new perspectives for the application of MSCs in Tissue Engineering (TE), with special interest for the pre-vascularization of cell dense constructs. In this work, cell sheet technology was used to create a scaffold-free construct composed of osteogenic, endothelial and perivascular-like (CD146+) cells for improved in vivo vessel formation, maturation and stability. The CD146 pericyte-associated phenotype was induced from human bone marrow mesenchymal stem cells (hBMSCs) by the supplementation of standard culture medium with TGF-b1. Co-cultured cell sheets were obtained by culturing perivascular-like (CD146+) cells and human umbilical vein endothelial cells (HUVECs) on an hBMSCs monolayer maintained in osteogenic medium for 7 days. The perivascular-like (CD146+) cells and the HUVECs migrated and organized over the collagen-rich osteogenic cell sheet, suggesting the existence of cross-talk involving the co-cultured cell types. Furthermore the presence of that particular ECM produced by the osteoblastic cells was shown to be the key regulator for the singular observed organization. The osteogenic and angiogenic character of the proposed constructs was assessed in vivo. Immunohistochemistry analysis of the explants revealed the integration of HUVECs with the host vasculature as well as the osteogenic potential of the created construct, by the expression of osteocalcin. Additionally, the analysis of the diameter of human CD146 positive blood vessels showed a higher mean vessel diameter for the co-cultured cell sheet condition, reinforcing the advantage of the proposed model regarding blood vessels maturation and stability and for the in vitro pre-vascularization of TE constructs.por
dc.description.sponsorshipFunding provided by Fundacao para a Ciencia e a Tecnologia project Skingineering (PTDC/SAU-OSM/099422/2008). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.por
dc.language.isoengpor
dc.publisherPLOSpor
dc.relationinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/99422/PT-
dc.rightsopenAccesspor
dc.subjectPericytespor
dc.subjectTissue engineeringpor
dc.titlePerivascular-like cells contribute to the stability of the vascular network of osteogenic tissue formed from cell sheet-based constructspor
dc.typearticle-
dc.peerreviewednopor
dc.relation.publisherversionhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0041051por
dc.commentshttp://www.3bs.uminho.pt/node/15870por
sdum.publicationstatuspublishedpor
oaire.citationStartPagee41051por
oaire.citationIssue7por
oaire.citationTitlePLoS ONEpor
oaire.citationVolume7por
dc.date.updated2013-04-10T10:07:01Z-
dc.identifier.doi10.1371/journal.pone.0041051-
dc.identifier.pmid22829909por
dc.subject.wosScience & Technologypor
sdum.journalPLoS ONEpor
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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