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TitleTransformation of a flocculating saccharomyces cerevisiae using lithium acetate and pYAC4
Author(s)Venâncio, Armando
Domingues, Lucília
Lima, Nelson
Issue date1999
JournalJournal of Basic Microbiology
Citation"Journal of basic microbiology". ISSN 0233-111X. 39:1 (1999) 37-41.
Series/Report no.1999;23
Abstract(s)A flocculating yeast Saccharomyces cerevisiae ura3 was transformed by the method based on treatment of intact cells with lithium acetate plus single-stranded carrier DNA using the shuttle vector pYAC4. The transformation efficiency was above 10³ transformants per microgram of plasmid DNA which is similar to other described yeast transformation systems. Under selective pressure, the transformed cells were stable and maintained the flocculation ability. Thus, this simple transformation system can be used for gene expression studies in flocculating yeasts, overcoming disadvantages of conventional methods such as the spheroplast one.
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series
DCILM - Artigos (Papers)

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